The steps for constructing a recombinant plasmid containing the gene for luciferase and inserting the plasmid into the bacterial cell are:
Use PCR to amplify the gene for luciferase.Perform restriction digestion of the luciferase gene and the plasmid.Ligate together the luciferase gene and the plasmid to generate a recombinant plasmid.Transform bacteria with the recombinant plasmid using electroporation.Plate the bacterial cells, and screen for positive transformants.Therefore, the correct order is D, A, C, B, and E
Plasmids are used by researchers to insert DNA from other sources into plasmids to form recombinant DNA molecules. Bacterial cells that have been transformed with recombinant plasmids will become recombinant bacteria.
Transformation is the process of introducing foreign DNA into a cell. Bacterial transformation with plasmids is important not only for studying bacteria but also because bacteria are used as a means of plasmid storage and reproduction. Therefore, nearly all plasmids (even those designed to be expressed in mammalian cells) contain both the bacterial origin of replication and antibiotic resistance genes for use as selectable markers in bacteria.
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__________ is a highly conserved nucleotide sequence that has been found in developmental regulatory genes in many diverse organisms.
Homeobox is a highly conserved nucleotide sequence that has been found in developmental regulatory genes in many diverse organisms.
A Homeobox (Hox) is a highly conserved nucleotide sequence that has been found in developmental regulatory genes in many diverse organisms. It is most commonly associated with the control of segment identity in animals and is composed of a 180-base pair DNA sequence that acts as a transcription factor, allowing it to control the expression of other genes. The Homeobox is the key component in the development of the body plan of the organism, with different Hox genes controlling the development of different parts of the body. The Homeobox is also involved in the regulation of plant development and is believed to be responsible for the vast range of morphological variation seen in plants. The Homeobox is an important evolutionary tool, allowing for the rapid adaptation of organisms to their environment.
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Calculate the surface area,
volume, and surface area to volume ratio of a
cube with 3mm sides.
surface area = 6 * (side length * side length) = 6 * (3mm * 3mm) = 54mm^2
Volume = (side length * side length * side length) = (3mm * 3mm * 3mm) = 27mm^3
Surface area to volume ratio = surface area / volume = 54mm^2 / 27mm^3 = 2
Therefore, the surface area of the cube is 54mm^2, the volume is 27mm^3, and the surface area to volume ratio is 2. I hope this helps! Let me know if you have any other questions.
Select the statements that accurately describe the differences between a hemiacetal and a glycoside. a. A glycoside forms when a hemiacetal condenses with an alcohol. b. A hemiacetal forms when an aldose condenses with an alcohol. c. An O-glycosidic bond does not involve an anomeric carbon atom. d. Hemiacetal formation eliminates a chiral center. e. Disaccharides contain glycosidic bonds.
The statement that accurately describes the differences between a hemiacetal and a glycoside is Hemiacetal formation eliminates a chiral center.
The correct option is D.
What are hemiacetals and glycosides?
Hemiacetals are compounds formed when the -CHO group an aldehyde reacts with the -OH group of an alcohol.
A common example of hemiacetals is sugar molecules such as glucose.
Glycosides are compounds formed when sugar monomers are bonded through their anomeric carbon to another group to form an O-glycosidic bond or an S-glycosidic bond. For example, starch molecules are glycosides formed from glucose monomers.
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what is biological (or biochemical) oxygen demand (bod)? how are excess nutrients in water related to bod?
The term "biochemical oxygen demand" (BOD) refers to how much oxygen is used by bacteria and other microorganisms during the aerobic decomposition of organic matter at a particular temperature.
One thing you cannot see in a lake's water when you look at it is oxygen. Temperature, pH, the type of microorganisms present, as well as the type of organic and inorganic material in the water, all have an impact on the rate of oxygen consumption. Wastewater from sewage treatment plants often contains organic elements that are degraded by microbes, which use oxygen in the process. If the B.O.D. is high, microbes are utilizing the majority of the oxygen. Larger aquatic species find it difficult to thrive in this environment. On the other hand, when the BOD is low, there is a surplus of oxygen, which results in good water quality.
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what research question did david tilman and colleagues address when they planted varying numbers of species from different functional groups of grassland plants in experimental plots?
It would allow a adequate comparison for erosion. Calculated soil depth through time on the a range of experimental plots with varied levels of species richness.
What did David Tilman demonstrate the relationship between species richness and community stability?He discovered plots in his experiment with much more species but less change in biomass production from prior years.He also came to the conclusion that greater diversity boosted production.Thus, David Tilman demonstrates that "the species richness of a community determines its stability."
What impact does species diversity have on productivity?If different species consume different resources, increasing the initial number of species in a system can lead to complementarity in resource usage.An assemblage of species' production will be higher in this situation than that of the productivity of the any one species.
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intestinal polyps are benign neoplasms and the first stage in development of colon cancer. these findings support the notion that: a. tumor invasion and metastasis progress more slowly in the gastrointestinal tract. b. apoptosis is triggered by diverse stimuli including excessive growth. c. an accumulation of mutations in specific genes is required for the development of cancer. d. cancers of the colon are more easily diagnosed in the benign form because they can be visualized during colonoscopy.
Cancer must emerge as a result of a buildup of mutations in particular genes.
Option c is accurate.
Which gene is an illustration of an oncogene?The involvement of the N-myc gene, which is linked to c-myc, in neuroblastoma is a well-known instance of oncogene amplification (a childhood tumor of embryonal neuronal cells).
How does the telomerase enzyme function in cancer cells?A molecule of RNA that functions as a template for new telomere segments is present in the enzyme. The majority of malignancies turn on the telomerase gene, which results in the telomeric repeat sequence being continuously appended to the ends of their DNA molecules.
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if a muscle fiber has a very long refractory period, what consequence would this have on the ability of the muscle to achieve a tetanic contraction?
When tetanized, the contracting withinside the muscle stays consistent in a consistent state.
This is the maximal viable contraction. During tetanic contractions, muscle mass can shorten, prolong or continue to be consistent period. Tetanic contraction is generally normal (together with whilst keeping up a heavy box). Contraction period - anxiety will increase as movement capacity is spreading alongside the period of the muscle tissue.
Relaxation period – muscle relaxes, relieves anxiety or comes again to its unique period. Since it takes place because of passive anxiety from the connective tissues, takes extra time than the contraction phase. The longer the muscle fiber, the bigger the pressure it may generate because of the elevated wide variety of myosin-actin bridges it may form.
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Secondary Succession: An ecosystem is _____________________ and is __________________ by a new one.
Answer: LIKE IT
Explanation:
hydrosere, xerosere or derosere.
What happens when fuels are burned?
When fuels are burned, A high-temperature exothermic redox chemical reaction takes place between a fuel and an oxidant, often atmospheric oxygen, producing oxidized, generally gaseous compounds in a mixture known as smoke.
Combustion is frequently a complex series of basic radical reactions. Endothermic pyrolysis converts solid fuels like wood and coal into gaseous fuels, whose combustion provides the heat needed to make more of them.
The heat produced by combustion is frequently intense enough to produce blazing or flame-like incandescent light. A basic example is the interaction of hydrogen and oxygen producing water vapor, which is often used to power rocket engines. This reaction produces 242 kJ/mol of heat and so decreases the enthalpy.
High oxidation potential oxidants for combustion include ambient or pure oxygen, chlorine, fluorine, chlorine trifluoride, nitrous oxide, and nitric acid. For example, hydrogen burns in chlorine to generate hydrogen chloride, with the characteristic heat and light liberation of combustion.
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Can I turn my cat into a diamond?
Answer:
Yes. It is scientifically possible to turn your beloved deceased pet or human relative into a diamond that you wear on your finger as a token of remembrance. In fact, there are companies that exist right now that are willing to do this for you.
Answer:
Yes. It is scientifically possible to turn your beloved deceased pet or human relative into a diamond that you wear on your finger as a token of remembrance. In fact, there are companies that exist right now that are willing to do this for you.
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Explanation:
if you cut a piece of dna with enzyme e, another piece with one of the enzymes you selected in the previous question, and ligate the two pieces with each other, can the resulting ligated dna be recut with enzyme e (at the position where the ligation occurred)?
If you cut a piece of DNA with enzyme e, another piece with one of the enzymes you selected in the previous question, and ligate the two pieces with each other, yes it can be the resulting ligated DNA be recut with enzyme e.
Deoxyribonucleic acid is a kind of DNA. DNA is a polymer made up of two polynucleotide chains that coil around one another to form a double helix. (listen);DNA) All known organisms and many viruses have genetic information in the polymer that is necessary for their development, operation, growth, and reproduction. Nucleic acids include DNA and ribonucleic acid (RNA). Nucleic acids are one of the four main categories of macromolecules that are necessary for all known forms of life, along with proteins, lipids, and complex carbohydrates (polysaccharides). Because the two DNA strands are made up of simpler monomeric units termed nucleotides, they are referred to as polynucleotides. Each nucleotide is made up of a phosphate group, a deoxyribose sugar, and one of the four nitrogen-containing nucleobases (cytosine [C], guanine [G], adenine [A], or thymine [T]). An alternating sugar-phosphate backbone is created when the nucleotides are linked together in a chain by covalent connections (also referred to as the phospho-diester linkage) between the sugar of one nucleotide and the phosphate of the next.
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consider the beetle pair in cross d of model 1. what is the probability for that beetle pair to have three black exoskeleton offspring in a row?
There's a about 75% chance that beetle couple will produce three successive offspring with black exoskeletons.
Some animal species' bodies are supported and shielded by hard coverings called exoskeletons. Exoskeleton literally translates to "outside skeleton." Animals without backbones known as invertebrates frequently have exoskeletons. Animals with exoskeletons most commonly include insects. Chitin is a material that serves as the exoskeleton of insects. Chitin is also a structural component of the exoskeletons of similar species such as scorpions, ticks, mites, spiders, lobsters, shrimp, and lobsters. Exoskeletons are rigid and hard, yet they also have joints or bending parts. Animals are able to move easily thanks to these joints. Land animals have spiracles, which are tiny breathing pores in their exoskeletons.
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Blue Water Power Corporation wants to begin operations that include the discharge of waste into navigable waters. Under the Clean Water Act, Blue must install certain equipment
Under the Clean Water Act, the company must install certain equipment before beginning operations.
The CWA expressly forbids any discharge of a pollutant from a point source to surface waters unless done in accordance with the act's requirements, and the statute has maintained as an explicit "national goal" since 1972 the elimination of all point-source discharges to surface waters by 1985.
The Water (Prevention and Control of Pollution) Act was enacted in 1974 to provide for the prevention and control of water pollution, as well as the maintenance or restoration of the country's water's wholesomeness. The Act seeks to maintain or restore the wholesome nature of water in order to establish Boards and vest them with the necessary powers to carry out the Act's purposes.
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What am I going to do or apply to protect and promote healthy sexuality?
Contraction fitness is an critical a part of bodily and intellectual fitness.
It is a key a part of our identification as humans collectively with the essential human rights to privacy, a own circle of relatives life, and dwelling unfastened from discriminatio .
Contraction fitness is a large a part of life. It can have an effect on and is tormented by different factors of fitness. This consists of bodily, intellectual, emotional, and social fitness.
Being in accurate contract fitness method you're properly informed, careful, and respectful to your self and others. Inform them approximately the terrible outcomes of youngsterager intercourse for each bodily and intellectual fitness. Also, tell them of the contract transmitted illnesses inclusive of aids, HIV, and so forth from such unprotected sexual activity.
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HELP PLEASE (PEOPLE THAT KNOW)
Answer:
The two ways of finding the age of a fossil are relative and absolute dating.In relative dating, paleontologists use the fossil's location in layers of rock to find its approximate age.Absolute dating allows paleontologists to know the actual age of a fossil through using a element called carbon-14.The element carbon-14 is important for paleontologists because it allows them to get an exact age of a fossil instead of just an approximation.Which of the following requires treatment with both antibiotics and antitoxins? A) diphtheria. B) tuberculosis. C) whooping cough. D) scarlet fever
Diphtheria requires treatment with both antibiotics and antitoxins.
Antibiotics are drug treatments that combat infections resulting from bacteria in humans and animals by using both killing the microorganism and making it difficult for the bacteria to develop and multiply. Microorganisms are germs. They live within the environment and all over the outside and inside of our bodies.
An antibiotic is a sort of antimicrobial substance lively towards microorganisms. It's miles the most important sort of antibacterial agent for fighting bacterial infections, and antibiotic medicinal drugs are widely used in the remedy and prevention of such infections.
Antibiotics are used to deal with or save you from some forms of bacterial contamination.
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What process is responsible for linking amino acids together to form polypeptides?
The process responsible for linking amino acids together to form polypeptides is called dehydration reactions.
Carbon, oxygen, hydrogen, & nitrogen make up amino acids. A water molecule is released as a result of the joining of two amino acids. Condensation is the name given to the process that joins two amino acids.
The dehydration process is another name for the condensation reaction. The carboxyl group supplies a hydroxyl group, whereas the amino group provides one hydrogen.
By joining the acetyl group with one amino acid as well as the carboxylate of another, together with the emission of a water molecule known as a peptide bond, a covalent connection is created. As a result, the sequence of amino acid residues is known as a polypeptide chain.
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Students are completing a lab in which they let a lab cart roll down a ramp. The students record the mass of the cart, the height of the ramp, and the velocity at the bottom of the ramp. The students then calculate the momentum of the cart at the bottom of the ramp.
A 4 column table with 3 rows. The first column is labeled Trial with entries 1, 2, 3, 4. The second column is labeled Mass of Cart in kilograms with entries 200, 220, 240, 260. The third column is labeled Height of ramp in meters with entries 2.0, 2.1, 1.5, 1.2. The fourth column is labeled Velocity at Bottom in meters per second with entries 6.5, 5.0, 6.4, 4.8.
Which trial’s cart has the greatest momentum at the bottom of the ramp?
trial 1, because this trial has the greatest velocity
trial 2, because this trial has the greatest ramp height
trial 3, because this trial has a large mass and a large velocity
trial 4, because this trial has the greatest mass
trial 3, because this trial has a large mass and a large velocity
d. plasmids which cell structure is an important component in modern genetic engineering techniques?
Plasmids, genes required for aging and metabolar activity. Cell structure is an important component in modern genetic engineering techniques.
Extrachromosomal DNA that is physically different from chromosomal DNA and has its own replication apparatus is called a plasmid. Small, circular, double-stranded DNA molecules known as plasmids are most usually found in bacteria, while they can also occasionally be detected in archaea and eukaryotic cells. Plasmids usually carry genes that give an organism a survival benefit and a selective advantage in the natural world, such as antibiotic resistance. Contrary to chromosomes, which are enormous and contain all the genetic information necessary for regular cellular function, plasmids are frequently quite small and only contain supplementary genes that may be useful in particular contexts or conditions. Artificial plasmids are often used in molecular cloning as vectors to encourage the replication of recombinant DNA sequences inside hosts. Plasmids are regarded as replicons, or DNA molecules that can replicate on their own in the appropriate host. Similar to viruses, plasmids are not commonly considered to be a component of life. Plasmids are typically transferred from one bacterial species to another by conjugation (even those of a different species). Plasmids are thought to be a part of the mobilome, and the transfer of genetic material from one host to another is one way that genes are transferred horizontally.
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Chronic inflammatory condition consisting of distinct red or pink lesions covered with silver scaling
a. True
b. False
Chronic inflammatory condition consisting of distinct red or pink lesions covered with silver scaling: Not contagious, thought to be an autoimmune disease.FALSE
Irritation is a vital part of your frame's recovery technique. It happens when inflammatory cells tour the location of a harmful or foreign body like bacteria. If inflammatory cells stay too lengthy, it may lead to continual inflammation.
The inflammatory reaction (infection) takes place when tissues are injured by way of microorganisms, trauma, pollutants, warmth, or every other purpose. The damaged cells launch chemical compounds consisting of histamine, bradykinin, and prostaglandins. these chemicals motivate blood vessels to leak fluid into the tissues, inflicting swelling.
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what condition involves a thickening of the lens of the eye, which causes vision to become cloudy, opaque, and distorted?
The condition is Eye Cataracts.
When injury or aging occurs, the tissues that make up the lens of the eye changes. The fibers and proteins in the lens begin to break down, making vision cloudy or blurry.
The haze thickens as the cataract continues to grow. Cataracts block light from reaching the lens and impair vision. People with cataracts lack sharp images in their vision.
This is because light cannot pass through the lens, which helps form a sharp image on the retina.
There are different types of cataracts: nuclear cataract (forms in the center of the lens), cortical cataract (forms in the rim of the lens), posterior subcapsular cataract (forms in the back of the lens), and congenital Cataracts (they are present at birth).
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in multiple organ dysfunction syndrome (mods), the gut hypothesis attempts to explain which phenomena?
in multiple organ dysfunction syndrome (mods), the gut hypothesis attempts to explain, translocation of bacteria.
The Multiple Organ Dysfunction Syndrome (MODS) is the emergence of a possibly reversible physiologic derangement affecting two or more organ systems that were unrelated to the condition that led to ICU admission, and that occurs after a physiologic insult that may be life-threatening.
It is a life threatening condition that can be termed as sepsis, leading to multi-organ failure.
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Hannah is trying to figure out how tooth size is determined in two different wolves. in wolf 1, the copies of the gene are different from each other. in wolf 2, the copies of the gene are the same as each other. how does this affect how many types of proteins there are in each tooth cell?
Hannah figure out tooth size in two different wolves:
Wolf 1 has two types of protein for the tooth size feature.
Wolf 2 has one type of protein for the tooth size feature.
Can the same proteins connect in different ways?
Even though the proteins for the stripe pattern are the same, the proteins can interact in various ways, thus they will have different striped patterns.
How do you separate proteins from the same size?
It is described how to separate proteins with virtually identical sizes but various isoelectric points. Convective electrophoresis is the technique employed, and it is based on how proteins are transported over a porous membrane while simultaneously being affected by diffusion, electrical migration, and opposing convective flow.
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Cell division that reduces chromosome number from diploid to haploid, reduction division is called?
Meiosis, also known as "reduced division," involves cutting the number of chromosomes in half to ensure that the baby's chromosomal count will be accurate after the fusing of the sperm and the egg.
Which sort of cell division causes the diploid number of chromosomes to change to haploid?Meiosis, a type of cell division in which the number of chromosomes in a parent diploid cell is cut in half, is the process that results in the production of haploid gametes.
Does mitosis turn haploid from diploid?In meiosis, diploid cells are divided into four new haploid cells, whereas in mitosis, diploid cells are mostly divided into two new diploid cells.
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Aphids can feed in leafy treetops or tree canopies. ants are usually not abundant in tree canopies unless aphids are also present. you introduce aphids to an area of the tree canopy and survey the abundance of ants over time. Finally, you take a tree and block the ants from the canopy. Then you spend three years counting the number and types of insects on the forest floor around this tree (tree A) and in the area around a tree in which ants are not blocked (tree B). You find that the area around tree A has 25% fewer ants and 40% more beetles than the area around tree B. These data support the hypothesis that: a. ecological interactions in the tree canopy always have a negative effect on forest floor communities. b. there is no relationship between ant abundance and beetle abundance. c. there is a negative relationship between ant abundance and beetle abundance. d. ecological interactions in the tree canopy have no effect on forest floor communities. e. Othere is a positive relationship between ant abundance and beetle abundance.
These data support the hypothesis that option c. there is a negative relationship between ant abundance and beetle abundance.
Aphids are little sap-sucking insects and members of the arthropod family. Common names embrace aphid and blackfly, though people inside a species will vary wide in color. The cluster includes the soft white woolly aphids. aphids will be quite damaging to plants, like crops, which implies they'll destroy our farms, trees, and gardens if left uncurbed.
In biology, the tree canopy is the surface portion of a plant cropping or crop, shaped by the gathering of individual plant crowns. In forest ecology, cover conjointly refers to the higher layer or environment zone, shaped by mature tree crowns and together with alternative biological organisms.
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What does hydrocarbon release?
Carbon dioxide (CO2) and other greenhouse gases are released by hydrocarbons, which contribute to air pollution and climate change. CO2 is an inevitable byproduct of the combustion of hydrocarbons.
Aside from the repercussions of burned hydrocarbons, unburned hydrocarbons are even more hazardous when they escape. Motor exhaust, as well as evaporating petroleum and gas, include toxic, carcinogenic compounds. Heavy forms can pollute groundwater and soil.
Because methane, the most discussed hydrocarbon in this context, is a more potent heat-trapping greenhouse gas than carbon dioxide, it has a bigger influence on global warming when it escapes into the atmosphere unburned.
It would be hard to completely stop using crude oil, gas, and coal, but we may try to lessen their negative impacts while developing the future's fully emission-free alternatives.
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of the choices below, which best describes the effect predation has on the predator/prey organisms involved in the relationship?
The easiest way to sum up how predation affects the species engaged in the predator-prey interaction is benefit—harmed.
Predation is a sort of relationship between two organisms in which one creature, known as the prey, suffers harm while another, the predator, benefits. Predators kill their victim and eat it in order to survive. For instance, when a lion hunts its target and kills the deer, the lion benefits because it gets to eat the animal, but the deer suffers since it lost its life. When resources are scarce and species compete to get them, competition occurs in an ecosystem. Predation is a term used to describe a particular form of competition in which a predator eats its victim.
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In animals, what is the difference between reproductive cloning and therapeutic cloning?
Reproductive cloning is the process of creating genetic copies of entire organisms, whereas therapeutic cloning is the process of creating genetic copies of tissues or organs to replace damaged body parts.
Reproductive cloning is the intentional creation of genetically identical individuals. Each newly created person is a clone of the original. Natural clones are monozygotic (identical) twins. Somatic cell nuclear transfer is used in reproductive cloning to create an animal that is genetically identical to a donor animal. The newly created embryo is placed back into the uterine environment where it can implant and develop in reproductive cloning. Dolly the sheep is probably the best-known example.
The transfer of nuclear material isolated from a somatic cell into an enucleated oocyte with the goal of producing embryonic cell lines with the same genome as the nuclear donor is known as therapeutic cloning. The process of creating a cloned embryo solely for the purpose of producing embryonic stem cells with the same DNA as the donor cell is known as therapeutic cloning. These stem cells can be used in experiments to better understand disease and develop new treatments.
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microbiology boiling water is a common method used to sterilize water that may be contaminated. subjecting microbes to such high temperatures is an example of a(n) technique
Boiling water is a common method used to sterilize water that may be contaminated. Subjecting microbes to such high temperatures is an example of a(n) microbicidal technique.
Any drug or method for killing germs (bacteria, viruses, and other microorganisms that can cause infection and disease). Also known as germicide.
Microbicidal antimicrobials kill microorganisms; microbiostatic antimicrobials only prevent their development. Bleach and other disinfectants are non-selective antimicrobials."
Is alcohol a microbicidal?
The FDA has not approved any liquid chemical sterilant or high-level disinfection that contains alcohol as its primary active component. These alcohols are bactericidal rather than bacteriostatic against vegetative bacteria; they are also tuberculocidal, fungicidal, and virucidal but do not kill bacterial spores.
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Full Episode :Boiling water is a common method used to sterilize water that may be contaminated. Subjecting microbes to such high temperatures is an example of a(n) _________________ technique.
a. microbicidal
b. microbistatic
c. intermittent sterilization
d. tyndallization
e. pasteurization
in eukaryotes, the cap on an mrna is the site where a. ribosomes attach at the start of translation. b. the mrna is protected from attack by rna-digesting enzymes. c. the start codon is located. d. the process of translation ends. e. the stop codon is covered until needed.
The correct option is A ; Ribosomes attach at the start of translation , The ribosome and a tRNA connect to the mRNA at the start of translation.
The tRNA is found in the first docking site of the ribosome. The anticodon of this tRNA is complementary to the beginning codon of the mRNA, where translation begins.
The cap is a guanine (G) nucelotide that protects the mRNA from degradation. It also assists the ribosome in attaching to the mRNA and beginning to read it in order to produce a protein.
The cap shields the 5′ end of the main RNA transcript from ribonuclease assault and is identified by eukaryotic initiation proteins involved in assembling the ribosome on mature mRNA prior to translation start.
All eukaryotic mRNA has a cap structure, which is a N7-methylated guanosine connected to the RNA's first nucleotide through .
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