what is the use of gel electrophoresis buffer?

Answers

Answer 1

Solution:

Remember that Electrophoresis separates macromolecules by size, charge, and other properties.

Buffers in gel electrophoresis are used to generate ions and thus carry a current flow (transmit a charge through the gel) Also, let's remember that a buffer is a substance that keeps pH levels stable. Therefore, buffers in gel electrophoresis are used to maintain the pH at a relatively constant value.


Related Questions

Hi I need help with this question please and thank you

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The process of transcription and translation is important since their regulation decides the genes expressed by a cell, determining the function that this cell can perform.

24. A phenotype that results from a dominant allele must have at least _________dominant allele(s) present in the parent(s).A. oneB. twoC. three

Answers

A: One

If an allele is dominant, it only needs one to be the phenotype.

Pictured below is a food web showing feeding relationships between organisms in the Chesapeake Bay. Which of the following species would contain the lowest amount of stored energy inthis ecosystem?A.GeeseB.PhytoplanktonC.Bald eagleD.Sea ducks

Answers

The energy flow in a food chain works from the highest to the lowest amount, since the energy flows starts from the light used by the producers to make photoshynthesis, and is gradually lost with the passafe of each trophic level. Therefore, between the organisms in the Chesapeake Bay the alternative C. Bald eagle is the one that presents the species with the lowest amount of stored energy in this food chain, being it the last trophic level (tertiary comsumer).

May I please get help with these analysis questions. I also need to provide examples for each questionI would appreciate it if I could please get help with answering them all in a way I can understand and get a good grade1) Consider the the specializations of the cells above. What are some advantages to being a multicellular organism as opposed to a unicellular organism?

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The advantages of multicellular organism over a unicellular organism is that:

- The multicellular organism can have a longer lifespan because they cells can die and be substitued without the death of the whole organism;

- The multicellular organism can increse in size without limitations because the cells integrate and associated;

- In addiction, multicellularity also permits each cell be able to specialize in different metabolic activities.

- The multicellularity promotes differents types of cells in one organism.

Shape is essential for an enzyme but?Shape is essential for an enzyme so?

Answers

answer and explanation

10. Which of the following are the reactants of photosynthesis?A. water and glucoseB. glucose and carbon dioxideC. carbon dioxide and waterD. oxygen and water

Answers

The correct option is C. carbon dioxide and water

Photosynthesis formula 6CO2 + 6H2O → C6H12O6 + 6O2.

The reactants are

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a rock is a natural substance composed of solid crystals of different materials that have been fused together into a solid lump.

Which options correctly describe the components of the scientific method?

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The scientific method is a series of steps followed during scientific investigations, with the objective of testing possible answers to the questions asked.

Some of the steps in this method are:

-Observations

-Questions

-Research of the background

-Hypothesis

-Testing of the hypothesis or experimentation

-Analysis of data

-Results

-Conclusions

We can compare the options with this.

a) That the results are believable is a somewhat ambiguous characteristic, so is not very useful to describe the components of the scientific method.

b) That the results can be verified or tested is indeed a characteristic of the scientific method, as the results should be replicable to be considered valid.

c) The measurement is important for the results to be objective, so this is also describing the scientific method.

d) It's not strictly necessary that what we are testing can be detected by senses, because in some cases the subjects of the scientific method cannot be observed directly by humans, but we can use equipment to be able to detect them.

So, in this case, B and C would be the correct answers.

Sliding Filament Theory
No unread replies.11 reply.
The sliding filament theory of muscle contraction is probably one of the most difficult physiological concepts to grasp in BIO 141. The purpose of this discussion board is to assist students with their understanding of how skeletal muscle contracts by writing about and discussing the topic.

For the initial post, please explain in your own words your understanding of the sliding filament theory beginning from when the calcium is released from SR until the sarcomere actually shortens. Be use to include all of the protein filaments and their role in the contraction. Also, is there anything that you are having difficulty understanding? If you have questions, ask them.

Answers

Two ground-breaking scientific publications explaining the chemical underpinnings of muscle contraction were published in 1954.

What is sliding filament theory?

The cylindrical, long cells that make up skeletal muscle. They are referred to as muscle fibers or myofibers because of how they appear. Skeletal muscle fibers are multinucleated cells, which means that they have multiple nuclei (rather than a single nucleus), as a result of the early developmental fusion of numerous precursor muscle cells (embryonic myoblasts).

Some parts of a sarcomere shrink while others remain the same size. A sarcomere is defined as the space between two adjacent Z discs or Z lines; this space is shrunk when a muscle contracts.

Only thick filaments are present in the H zone, which is the center of the A zone, and it shortens during contraction. The I band includes just thin filaments and likewise shortens.

Therefore, Two ground-breaking scientific publications explaining the chemical underpinnings of muscle contraction were published in 1954.

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Which structure feature of the cell membrane allows molecules such as oxygen and carbon dioxide to diffuse into and out of the cell

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Answer: The lipid bilayer


The structure of the lipid bilayer allows small, uncharged substances such as oxygen and carbon dioxide, and hydrophobic molecules such as lipids, to pass through the cell membrane, down their concentration gradient, by simple diffusion.

I need help with this can not find answers anywhere !

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Acromial end of the clavicle articulates with the acromion of the scapula.

Head of the radius articulates with the capitellum of the humerus.

Sternal end of clavicle articulates with manubrium of sternum.

Trochlea of humerua articulates with troclhea notch of ulna.

Glenoid cavity articulates with the head of humerus.

The point at which two or more bones meet is referred to as articulation or a joint which are functioning for movement and balance.

Control of gene expression in eukaryotic cells occurs at which level?

a. only the transcriptional level
b. epigenetic and transcriptional levels
c. epigenetic, transcriptional, and translational levels
d. epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels

Answers

Option D epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels regulate gene expression in eukaryotic cells.

Epigenetic modifications effect gene expression and have the ability to "turn on" and "turn off" genes, whereas hereditary changes can affect which proteins are created. Since your environment and habits, including diet and exercise, can modify your epigenome, the relationship between your genetics, actions, and environment is easy to comprehend. Epigenetic mechanisms, which regulate how an organism's genome is influenced by its environment, are molecular processes .During transcription, translation, and subsequent protein modification, which normally leads to the production of protein, the genetic information is transferred from the archival copy of DNA to short-lived messenger RNA. Even though every cell in an organism has essentially the same DNA, the qualitative and quantitative changes in gene expression as well as the control of gene expression lead to the diverse cell types and functions.

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There are two parts to this question can I get a coherent explanation for this pls?

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A)In this case, we are talking of nicotinic and muscarinic receptors, in the first case the nicotinic is called like that because nicotine can activate them, meanwhile the second is not, so in the table type one would be nicotinic meanwhile type two would be muscarinic. So nicotinic receptors are ligand-activated receptors, they possess hetero-oligomers of five subunits each with four transmembrane domains, meanwhile the muscarinic are coupled to a G protein, consist of individual proteins with seven transmembrane domains.

Nicotinic receptors allow the entrance of NA+ ions or the exit of K+, In some other cases allow the pass of Ca+, depolarizing the cell and generating a postsynaptic potential. Meanwhile, the muscarinic receptors mediate the subsequent hyperpolarization (recovery of the neuron after depolarization) and a type of depolarization (slow polarization).

B)Actilcholinesterase hydrolyzes the acetylcholine and that way ends the action of the neurotransmitter released in the synaptic cleft, if this neurotransmitter is not eliminated the ionic channels mediated by the receptors would not open again, as long as the acetylcholine is still detected once depolarization occurred the channel will not open, so muscular contraction cannot happen normally if acetylcholinesterase is inhibited.

Need to know which is the correct answer about the trailing edge for cell bio

Answers

Answer:

The answer is the first option. The trailing edge is composed of many filopodia containing tight parallel bundles. These filopodia push trailing edge away from the location it was previously occupying.

Explanation:

Cells migrating in tissues exhibit both filopodia and lamellipodia. These thin protrusive extensions are ideal for exploring and penetrating tissue spaces. They are also well suited for intercalating between cells, such as during the migration of leukocytes across endothelial layers.

Therefore the answer is the first option.

. GENETICS
- Genes and heredity
Identification of dominant and recessive traits
Identification of the genotype and phenotype
Identification of the homozygous and heterozygous
What do Punnet square represent? How to set up a Punnet square correctly?

Answers

In order to determine whether a trait is dominant or not, you must have an organism that is known to be heterozygous. Parental phenotype that occurs in a heterozygous organism is dominant. Trait that reappear in f2 generation is recessive .

Identification of genotype of an organism with a dominant phenotype, one Test cross must be done .

If an organism exhibiting a dominant trait is homozygous or heterozygous for a specific allele, It is needed to  perform a test cross.

Punnett square is a table in which all of the possible results for a genetic cross between two individuals with known genotypes is performed .

Punnett Square, the genotype of each parent is placed on the sides of a four chambered box. Alleles of one parents is set up on top and other placed down one side .

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In which landscape region would you most likely find intensely metamorphosed rock?

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The New York State landscape is most composed by metamorphosed rocks as well the gneiss, which are rocks formed when subjected to high heat, pressure, hot mineral fluids. These types of rocks and condictions are found deep into the Earth and in the place where tectonic plates commonly meet.

What is the single source of both electrons used by AND the oxygen created by photosynthesis? During which part of the process does this occur?

Answers

Water is the single source as it provides the electrons by splitting into oxygen and hydrogen. This process happens during the light-dependent reactions.

While analyzing a karyotype, geneticists identify a missing chromosome. Which term describes this chromosomal abnormality?aneuploidypolyploidytriploidytetraploidy

Answers

The correct answer is the first one, aneuploidy. Aneuploidies refer to qualitative or quantitative abnormalities in the chromosome set.

Answer:

A, aneuploidy

Explanation:

Got it right on edge 2022 .

11. Which of these is a food chain from the diagram? A. Grain, rabbit, owlB. carrot, mouse, fox c. fox, bird, graind. grass, grasshopper, owl12. in the diagram above, where does ALL the energy come from?a. sunb. producerc. primary consumerd. secondary consumer 13. Which of those above would have the FEWEST number of organisms?a. grassesb. mousec. rabbits d. foxes

Answers

The organism in the diagram that can be considered a producer is the Grain,

Endotherms may use all the internal means to cool their temperature except which? A. Changes in blood flowB. PantingC. SweatingD. Sunning themselves

Answers

The correct answer is D. Sunning themselves

Sunning or sunbathing is a method used by the organism to raise its body temperature.

The options changes in blood flow, panting, and sweating are means of the endotherms to cool their body temperature.

In what stage of growth can hair most readily be removed from the scalp?

Answers

Answer:

The telogen phase

Explanation:

In the telogen phase, the follicle is dormant or resting. The transition period between the anagen and telogen phases is referred to as the catagen phase. Hairs are routinely lost during the telogen phase and often become a primary source of evidentiary material.

Draw a Punnett square to help you: A woman who is a carrier marries a normal man.a) Whats is the probability that their children will have hemophilia?b) What sex will a child in the family with hemophilia be?

Answers

Hemophilia is a genetic disorder that makes coagulation slower, which causes the person to bleed easily and for a more prolonged time.

The gene involved is located in the X hromosomes, so females are more likely to be just carriers, because they have two copies of the gene, while males that inherit the defective gene have only one copy, so they express the disease.

If a carrier woman has children with a healthy man, the Punnett square would be as follows:

This means that there i a 525 chance off having a daughter that is a carrier, a 25% chance ofa healthy daughter, a25% chance of a healthy son, and a 25% chance of a son w th hemophilia.

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Scientists believe that _______ of species that once existed on Earth are now extinct.A) about 40%B) about three-quartersC) nearly halfD) over 99 percent

Answers

Scientists believe that over 99 percent (D answer option) of species that once existed on Earth are now extinct. This can be known by archeologist research, which allows us to know what species lived in the past by studying the soil, its different layers, and the found fossils, dating with high precision how many years ago those organisms inhabited our planet. With these research tools, we have known that there were at least five massive extinctions on the Earth, where aquatic and terrestrial species perished, and they are the Ordovician-Silurian (around 440 mya), Devonian, the Permian-Triassic, the Triassic-Jurassic, and the Cretaceous-tertiary (65 mya) extinctions.

On a piece of paper (or anything you can use to solve this) complete a monohybrid cross for Rr with Rr.how many of the offspring will be RR?

Answers

Only 1 out of four individuals from the offspring of a monohybrid cross would be RR.

which of the cell is concerned with cellular metabolism?
a. nucleus
b. mitochondria
c. chloroplast
d. nucleolus​

Answers

As mitochondria is responsible for cellular metabolism that is why, according to the question mitochondria is the correct answer.

Mitochondria is a cell organelle, originally derived from a theory called endosymbiotic theory. According to this theory, mitochondria went through endosymbiosis that is mitochondria existed as an individual bacterium and was engulfed by a higher-level cell.

Mitochondria is known as the powerhouse of the cell, as it produces all the metabolites for the cellular respiration and metabolism.

Mitochondria produces ATP by number of metabolic pathways such as - glycolysis, where the breakdown of glucose takes place, and it is metabolized into pyruvate. Tri-carboxylic acid cycle or citric acid cycle also known as Kreb's cycle, an eight-step pathway that uses the pyruvate from glycolysis and converts into acetyl Co-A, which gives NADH and FADH2. Electron transport chain, that uses the NADH to transport electrons from highly electronegative complex to electropositive complex.  

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Rocky Mountain spotted fever is caused by _____. a virus bacteria rickettsia

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Answer:Rocky Mountain spotted fever is caused by Rickettsia rickettsii

Explanation:This fever is spread to humans by bites from the American dog tick or the wood tick.

Ticks, a infected tick with this bacterial disease.

I need help, look at the pic provided, it’s from my prep guide

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The chocolate chip, which represents fossil fuels, leave a hole or spaces when extracted from the cookie. Much like when we take fossil fuel underground, there will be empty spaces underground left behind when the fossil fuels are extracted. In the case of coal mine, there are surface mines. Surface mines level off mountains to extract the coals within. It can cause change of landscapes.

In the cookie's case, it looked ugly without the chocolate chip. In the case of the environment, the surface of the mountains are stipped off and are no longer capable of sustaining life.

7) Tom received $400 for his birthday, and he decided to spend it all. How much did he spend on clothes?

x

A. $96

B. $86

C. $75

D. $60

E. $20

x

8) How much did Tom’s snowboard cost?

x

A. $216

B. $180

C. $110

D. $85

E. $56

x

9) How much did he spend on video games?

x

A. $3

B. $14

C. $56

D. $86

E. $110

x

10) About how much did he spend on DVDs and video games combined?

x

A. $17

B. $34

C. $55

D. $68

E. $88

help!! please n thank u sm text my number so i can thank you sm! 785-580-8256

Answers

Answer:

7) A. $96

8) A. $216

9) C. $86

10) E. $88

Explanation:

7) Clothes = hat (3%) + sneakers (21%)

Hat: $400 * 3%(0.03) = $12

Sneakers: $400 x 21%(0.21) = $84

$12 + $84 = $96!

8) Find snowboard percentage by:

100% - (The all revealed percent)

= 100% - (21%+14%+8%+3%) = 51%

= $400 * 51%(0.51) = $216

9) Video games = 14%

= $400 * 41%(0.41) = $86

10)

Dvds: $400 * 8% = $32

Video games = $86

= $32 + $86

-----

I hope this is helpfull and understandable. Just ask me if u don't understand something about this ^^

According to historians, when did humans FIRST become interested in fitness?
(1 point)
O a few years before the first Olympics
O ever since the beginning of civilization
after the Industrial Revolution
O once the British Empire spread across the world

Answers

According to historians, when did humans first become interested in fitness ever since the beginning of civilization after the Industrial Revolution.

When we look back at human history, there seems to be a correlation between industrial developments and fitness. Early man had no means of propulsion. Food was a task that required walking, running, and abilities that the majority of people in this modern period can hardly even imagine. Back then, what we now refer to as exercise was simply a way of life.

Our bodies started to change in the past when man made the transition from a nomadic existence as a hunter to a sedentary one as a farmer. Despite the work, only specific body areas would receive workout, and the activities became repetitious.

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Why does genetic drift affect a
small population more than a
large one?
A. small populations have more diversity than a
large population
B. the loss of a few alleles causes a large shift in
the allele frequency of a small population
C. large populations have more allele diversity
so a dramatic event would result in more loss

Answers

Answer:

B

Explanation:

Genetic drift is more important in small populations because the chances of an allele being lost or fixed in the population are much higher, this is because each individual in a small population represents a larger proportion of the entire population (than in a large population).

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