The ADG1(ADP glucose pyrophosphorylase 1) is involved in flowering timing in plants. You are interested in developing a DNA probe using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu 1. determine the possible antisense oligo nucleotides possible from this amino acid sequence 2. How many total unique different oligonucleotide would need to be produced to cover all possibilities

Answer:

1)  UUC-AUG-AUC-AAG-UAA.

2) 48

Explanation:

1) Determine the possible antisense oligo nucleotides possible from this amino acid sequence

Given that the peptide sequence is = Glu-Glu-His-Asp-Val-Leu 1.

we will use a codon table to show the codons and Anticodons for each of the Amino acids  

Amino Acid   Codons                                                         Anticodons

 Glu                GAA, GAG                                                    UUC, CUC

 His                 CAU, CAC                                                    AUG, GUG

 Asp         GAU, GAC                                                    AUC, GUC

 Val                 GUU, GUC, GUA, GUG                             AAG, GAC, UAC,CAC

 Leu   UUA, UUG, CUU, CUC, CUA, CUG  | UAA, CAA, AAG, GAG,UAG,CAG

From the table above

The possible antisense oligo nucleotide sequence is : UUC-AUG-AUC-AAG-UAA.

2)  How many total unique different oligonucleotide would need to be produced to cover all possibilities

A desired peptide sequence requires 16 anticodons

Hence the total unique different oligonucleotide needed to be produced to cover all possibilities = 16 *3 = 48

The ADG1(ADP glucose pyrophosphorylase is involved in flowering timing in plants and a DNA probe is made by using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu.

1. To determine the possible antisense oligonucleotides possible from this amino acid sequence:

Since, for every amino acid, there is a specific codon and anticodons are present so according to the given information, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA.

2) The total number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48

since the amino acid sequence is Glu-Glu-His-Asp-Val-Leu has 16 anticodons and each anticodon have 3 nucleotides so the possible number of oligonucleotides are  :

16×3 = 48.

A codon is a trinucleotide sequence of RNA that corresponds to a specific amino acid.

An anticodon is a trinucleotide sequence complementary to that of a corresponding codon in a messenger RNA sequence.

Thus, according to codon and anticodon, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA and a number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48.

To learn more about the codon and anticodon click here:

https://brainly.com/question/19884283

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