What are the advantages and disadvantages of the serial dilution-agar plate procedure?​

Answers

Answer 1

Answer:

Advantages

- Serial dilution is used in microbiology to estimate the concentration or number of cells/organisms in a sample to obtain an incubated plate with an easily countable number of colonies.

- In biochemistry, serial dilution is used to obtain the desired concentration of reagents and chemicals from a higher concentration.

- In pharmaceutical laboratories, serial dilution is performed to receive the necessary concentration of chemicals and compounds as this method is more effective than individual dilutions.

- In homeopathy, homeopathic dilutions are used where a substance is diluted in distilled water or alcohol. It is believed than dilution increases the potency of the diluted substance by activating its vital energy.

Disadvantages

- An error might occur during the propagation of the sample, and the transfer inaccuracies lead to less accurate and less precise transfer. This results in the highest dilution to have the most inaccuracies and the least accuracy.

- Because serial dilution is performed in a stepwise manner, it requires a more extended period of time which limits the efficiency of the method.

- Serial dilution only allows the reduction of bacteria/cells but not the separation of bacteria/cells like in other techniques like flow cytometry.

- This technique also requires highly trained microbiologists and experts in aseptic techniques.


Related Questions

y= 4/3x -8
4x-3y=24

Answers

Answer:

(x,y)= (x, 4/3x-8)

Explanation:

Explain how and why different parts of a plant are sinks for the translocation of sucrose at different times of the year in a country where there are season, such as Russia ​

Answers

Answer:

The sucrose is transported around the plant in phloem vessels. It needs to be able to reach all cells in the plant so that the sucrose can be converted back into glucose for respiration. The movement of sucrose and other substances like amino acids around a plant is called translocation .

Belowground organs of plants (e.g. roots and rhizomes) are sinks during plant growth since they cannot perform photosynthesis. Some organs are both a source and sink. Leaves are sinks when growing and sources when photosynthesizing

define carbonization​

Answers

Answer:

It is the conversion of organic matters such as plants and animals remains into carbon through destructive distillation.

Answer:

carbonization is the term for the conversion of an organic substance into carbon or a carbon- containing residue through phrolysis or distructive distillation.

Explanation:

It can be carbonized extremely quickly, turning it into solid carbon

Ocean currents store energy and help to distribute heat and

Answers

Answer:

One way that the world’s ocean affects weather and climate is by playing an important role in keeping our planet warm. The majority of radiation from the sun is absorbed by the ocean, particularly in tropical waters around the equator, where the ocean acts like a massive, heat-retaining solar panel. Ocean water is constantly evaporating, increasing the temperature and humidity of the surrounding air to form rain and storms that are then carried by trade winds. In fact, almost all rain that falls on land starts off in the ocean. The tropics are particularly rainy because heat absorption, and thus ocean evaporation, is highest in this area.

Major current systems typically flow clockwise in the northern hemisphere and counterclockwise in the southern hemisphere, in circular patterns that often trace the coastlines.

Ocean currents act much like a conveyor belt, transporting warm water and precipitation from the equator toward the poles and cold water from the poles back to the tropics.

Explanation:

One way that the world’s ocean affects weather and climate is by playing an important role in keeping our planet warm. The majority of radiation from the sun is absorbed by the ocean, particularly in tropical waters around the equator, where the ocean acts like a massive, heat-retaining solar panel. Land areas also absorb some sunlight, and the atmosphere helps to retain heat that would otherwise quickly radiate into space after sunset.

The ocean doesn't just store solar radiation; it also helps to distribute heat around the globe. When water molecules are heated, they exchange freely with the air in a process called evaporation. Ocean water is constantly evaporating, increasing the temperature and humidity of the surrounding air to form rain and storms that are then carried by trade winds. In fact, almost all rain that falls on land starts off in the ocean. The tropics are particularly rainy because heat absorption, and thus ocean evaporation, is highest in this area.

Outside of Earth’s equatorial areas, weather patterns are driven largely by ocean currents. Currents are movements of ocean water in a continuous flow, created largely by surface winds but also partly by temperature and salinity gradients, Earth’s rotation, and tides. Major current systems typically flow clockwise in the northern hemisphere and counterclockwise in the southern hemisphere, in circular patterns that often trace the coastlines.

Ocean currents act much like a conveyor belt, transporting warm water and precipitation from the equator toward the poles and cold water from the poles back to the tropics. Thus, ocean currents regulate global climate, helping to counteract the uneven distribution of solar radiation reaching Earth’s surface. Without currents in the ocean, regional temperatures would be more extreme—super hot at the equator and frigid toward the poles—and much less of Earth’s land would be habitable.

What would the complementary strand of GTCCGTAACGTAT be?

Answers

Answer:

CAGGCATTGCATA

Explanation:

Given: GTCCGTAACGTAT

Under Chargaff's rule, Adentine is equal to Thymine, and Cytosine is equal to Guanine. (A=T, C=G)

Can anyone solve this

Answers

A habitat, Is the physical area where an organism lives.

Populations that experience exponential growth have unlimited sources. A population can not continue to grow
exponentially indefinitely. Eventually, the population of a species will reach their limit. What curve will occur when a
population has reached this limit and what are some possible reasons for this curve shape?
PLSSS HELPPP

Answers

Answer:

Carrying capacity

Explanation:

Carrying capacity is the limit to which a population can grow with the resources (food, water, oxygen, habitat space, etc.) are available to support them. Once they reach the maximum population that the environment can support, they reach their carrying capacity.

What evidence do we have that all continents were merged into one super-continent called Pangaea 250 million years ago?

Answers

Glacial deposits, specifically till, of the same age and structure are found on many separate continents that would have been together in the continent of Pangaea. Fossil evidence for Pangaea includes the presence of similar and identical species on continents that are now great distances apart.

what is a example of acceleration?

Answers

Answer:

For example, if a car turns a corner at a constant speed, it is accelerating because its direction is changing. The quicker you turn, the greater the acceleration. So there is an acceleration when velocity changes either in magnitude or in direction, or both.

Explanation:

hope this helps... (i can't see the pic...)

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The process of mitosis deals with?
Will mark brainliest

Answers

Answer: Gametes

Explanation:

Answer:

Gametes

Explanation:

When a cell divides by way of mitosis, it produces two clones of itself, each with the same number of chromosomes. When a cell divides by way of meiosis, it produces four cells, called gametes.

With the above paragraph one can conclude that mitosis deals with gametes.

What regulates breathing rate and how does the body sense when to breath?

Answers

Answer:

Involuntary respiration is controlled by the respiratory centers of the upper brainstem (sometimes termed the lower brain, along with the cerebellum). This region of the brain controls many involuntary and metabolic functions besides the respiratory system, including certain aspects of cardiovascular function and involuntary muscle movements (in the cerebellum).

Explanation:

The respiratory centers contain chemoreceptors that detect pH levels in the blood and send signals to the respiratory centers of the brain to adjust the ventilation rate to change acidity by increasing or decreasing the removal of carbon dioxide (since carbon dioxide is linked to higher levels of hydrogen ions in blood).

what is monosachrayde​

Answers

Basically any type of sugar (glucose for example) that cant be broken down to a simpler sugar

Answer:

A monosaccharide is the most basic form of carbohydrates. Monosaccharides can by combined through glycosidic bonds to form larger carbohydrates, known as oligosaccharides or polysaccharides.

Explanation:

A monosaccharide is a type of monomer, or molecule that can combine with like molecules to create a larger polymer.

What is one characteristic that a star’s spectrum can reveal?
A.
its direction
B.
its temperature
C.
when it was formed
D.
its constellation

Answers

Answer:

B. Temperature

how can strong emotions influence the decisions you make.

Answers

Answer:

Strong emotions can make you do many things (sometimes things you don't want to). When someones is very angry they usually act out of anger and when someone is very sad they usuallu act out of sadness. It takes a strong minded person to be able to control their emotions whithout ever acting out.

Explanation:

what the other person said was 100% true

Describe at least two abiotic and two biotic factors found in this biome. (Desert Biome) How
do these abiotic factors interact with the biotic factors?

Answers

Abiotic: the sunlight and water.

Biotic: Plants and insects

Explanation:

Plants such as cactuses can absorb and conserve water in their roots and use the sunlight to grow taller

Insects such as Desert beetles can trap moisture in their thick exoskeletons so they are parched or burned by the sun. They sometimes bury themselves deep in the sand where it's cooler.

The abiotic factors in the desert help the biotic factors survive

when you mix instant coffee creamer sugar and hot water all together what kind of mixture are you going to form​

Answers

Answer:caffe

Explanation:

Which process BEST explains how DNA is replicated?
A)
DNA is conservative. The original strands of nucleotides do not separate. A
copy composed of two new strands containing all of the new DNA base
pairs is produced to come together.
DNA is semi-conservative. The original strands of nucleotides do not
separate. A copy composed of two new strands containing all of the new
DNA base pairs is produced to come together.
B)
DNA is semi- conservative. The original strands of nucleotides do not separate. A copy composed of two new strands containing all of the new DNA base pairs is produced to come together.
C)
DNA is semi-conservative. The two strands of nucleotides separate. It
produces two copies of the original DNA molecule, each of which contains
one of original strand, and one new strand.
D)
DNA is dispersive. The original DNA chain breaks and recombines in a
random fashion before the double helix structure unwinds and separates to
act as a template for messenger RNA synthesis.

Answers

Answer:

C)

DNA is semi-conservative. The two strands of nucleotides separate. It

produces two copies of the original DNA molecule, each of which contains

one of original strand, and one new strand.

The statement that best explains the process of replication is statement C.

What do you mean by DNA replication?

DNA replication may be defined as the process by which a molecule of DNA is duplicated, where a parental strand gets divided into two daughter strands.

DNA is semi-conservative. The two strands of nucleotides separate. It produces two copies of the original DNA molecule, each of which contains one of the original strand, and one new strand.

Therefore, the statement that best explains the process of replication is statement C.

To learn more about DNA replication, refer to the link:

https://brainly.com/question/860995

The dispersal of food coloring in a beaker of water is an example of what process?

Answers

Answer:

this is an example of the process DIFFUSION

A calorie is the amount of energy it takes raise the... of 1 mL of water by 1° C.


a) temperature
b) mass
c) volume
d) density

Answers

The answer is a temperature.

How has biotechnology
contributed positively to
our lives?

Answers

Answer:

It has made genetically modified crops and farms to make farming more efficient and productive

Explanation:

Sleep is an important need for people, explain sleep and four
different stages of sleep.​

Answers

Answer:

Stage 1 non-REM sleep is the changeover from wakefulness to sleep. ...

Stage 2 non-REM sleep is a period of light sleep before you enter deeper sleep. ...

Stage 3 non-REM sleep is the period of deep sleep that you need to feel refreshed in the morning. ...

REM sleep first occurs about 90 minutes after falling asleep.

Explanation:

Sleep

Sleep is an important part of your daily routine—you spend about one-third of your time doing it. Quality sleep – and getting enough of it at the right times -- is as essential to survival as food and water. Without sleep you can’t form or maintain the pathways in your brain that let you learn and create new memories, and it’s harder to concentrate and respond quickly.

Stages of sleep

stage 1 non-REM sleep is the changeover from wakefulness to sleep. During this short period (lasting several minutes) of relatively light sleep, your heartbeat, breathing, and eye movements slow, and your muscles relax with occasional twitches. Your brain waves begin to slow from their daytime wakefulness patterns.

Stage 2 non-REM sleep is a period of light sleep before you enter deeper sleep. Your heartbeat and breathing slow, and muscles relax even further. Your body temperature drops and eye movements stop. Brain wave activity slows but is marked by brief bursts of electrical activity. You spend more of your repeated sleep cycles in stage 2 sleep than in other sleep stages.

Stage 3 non-REM sleep is the period of deep sleep that you need to feel refreshed in the morning. It occurs in longer periods during the first half of the night. Your heartbeat and breathing slow to their lowest levels during sleep. Your muscles are relaxed and it may be difficult to awaken you. Brain waves become even slower.

REM sleep first occurs about 90 minutes after falling asleep. Your eyes move rapidly from side to side behind closed eyelids. Mixed frequency brain wave activity becomes closer to that seen in wakefulness. Your breathing becomes faster and irregular, and your heart rate and blood pressure increase to near waking levels. Most of your dreaming occurs during REM sleep, although some can also occur in non-REM sleep. Your arm and leg muscles become temporarily paralyzed, which prevents you from acting out your dreams. As you age, you sleep less of your time in REM sleep. Memory consolidation most likely requires both non-REM and REM sleep.

Why do mutations code proteins?

Answers

Answer:

A nonsense mutation is a genetic mutation in a DNA sequence that results in a shorter, unfinished protein product. DNA is a chain of many smaller molecules called nucleotides. During protein formation, DNA (or RNA) nucleotide sequences are read three nucleotides at a time in units called codons, and each codon corresponds to a specific amino acid or stop signal (stop codon).

Explanation:

I will mark brainiest for correct answer

A pelican has a dip netting beak. How does it eat?

A. It traps fish between its upper and lower mandible much like a scissor,
B. It takes a big gulp of water with fish and filters the water off of the fish before swallowing,
C. It opens holes in trees and eats insects beneath the surface of the wood,
D. It burrows in the mud to find insects and other small invertebrates beneath the surface

Answers

Answer:

b is correct

Explanation:

Answer:

b they scoop them and then drain the water a swallow all remaining animals

Help is much needed please, thank you?

Answers

Answer:

I belive the answer is #3

Explanation:

Long-distance signalling

What will lead to changes in a phenotype of an individual?
a. medicines
b. nutrition frequency
c. DNA sequence variation
d. all of the above

Answers

Answer: D

Explanation:

PLEASE PLEASE PLEASE HELP AND GIVE EXPLANATION TOO!

FOR EXAMPLE: THE TYPE OF HEAT TRANSFER IS RADIATION BECAUSE...

*I ALREADY WROTE THE TYPE OF TRANSFER DOWN I JUST NEED EXPLANATIONS*


1.) Candle - The type of heat transfer here is radiation because...


2.) Tea Kettle & Mugs - The type of heat transfer here is conduction/convection because...


3.) Lamp - The type of heat transfer here is radiation because...



7.) Toaster - The type of heat transfer here is conduction/convection/radiation because...


9.) Radiator/Heater - The type of heat transfer here is radiation because...

Answers

Explanation:

1. a burning candle enits radiation in the form of heat and light, this is because of it emission of energy in rays or waves

2.tea and kettle mugs transfer heat by the actual movement of matter. heat leaves the nug as the currents of the steam and air rise. This also emmits heat to the surrounding area.

3.Radiation refers to the emission of energy in rays or waves. In this, a lamps energy is emmiting rays of heat the expands its heat and light to the surrounding area.

7. the coils in the toaster heat the air which heats the toast. A toaster uses infrared radiation because when you put food in the coild glow red.

9. this is beacuse, low wavelength, non visable infared radiation can carry heat directly from warm objects to cooler objects. It emmits and expands its heat energy to the surrounding area and other objects.

A scientist found an igneous rock that contained pyroxene and plagioclase. What would best help the scientist to correctly classify the rock?

A. Identifying the rock as either intrusive or extrusive

B. Observing the color of the rock based on minerals

C. Knowing the additional minerals found in the rock

D. Determining the amount of pyroxene in the rock

Answers

B.observing the color of the rock based on minerals

When we consider the motion of particles such as the molecules that comprise air, which of the following is False?

Group of answer choices

Molecules move in a random motion and will collide with other particles or a container

As temperature increases, there is an increase in molecular motion

Molecules move from areas of high concentration to areas of lower concentration

As temperature decreases, there is an increase in molecular motion

Answers

Answer:

D

Explanation:

If you see two contradictory answers (B & D), one has to be the choice. As temperature increases, molecular motion decreases is false. Diffusion is what choice C is describing and is true.

Which component of weather results in stormy, cloudy weather?

A. High pressure
B. Low pressure
C. High temperature
D.Low temperature

Answers

Answer: B. Low pressure

Explanation: Low-pressure areas are places where the atmosphere is relatively thin. Winds blow inward towards these areas. This causes air to rise, producing clouds and condensation. Low-pressure areas tend to be well-organized storms.

Low pressure component of weather results in stormy, cloudy weather. Therefore, option (B) is correct.

What causes cloudy and stormy weather?

Low pressure systems in the atmosphere are often to blame for the weather patterns of cloudy and stormy conditions. When there is low pressure in an area, it causes the air pressure to rise, which can result in the development of clouds and the production of precipitation. Low pressure systems have a tendency to produce air that is unstable, which can lead to severe weather such as thunderstorms, hurricanes, and other forms of severe weather.

In contrast, high pressure systems almost always result in weather that is free of clouds and precipitation. This is because the air that surrounds the high pressure area is sinking and remaining relatively still. The formation of gloomy and stormy weather can also be influenced by a variety of other factors, including temperature, humidity, and the direction and speed of the wind.

Learn more about cloudy weather, here:

https://brainly.com/question/1408324

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Union of haploid and diploid gamete results in a zygote that is

Answers

Answer:

Triploid

Explanation:

The process whereby gametes produced by different sexes of the same species of organism come together is called FERTILIZATION. A gamete is said to be HAPLOID (n) if it contains one set of chromosomes while it is DIPLOID (2n) if it contains two sets of chromosomes.

Hence, if an haploid (n) and diploid (2n) gamete unites, a zygote that is TRIPLOID (3n) emerges or forms. A triploid zygote will have three sets of chromosomes i.e. n + 2n = 3n.

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