Two distinct bands appear in a single lane at the completion of an SDS-PAGE analysis of an unknown protein. Select one: a. These bands represent two subunits of a homodimer. b. Each band represents a different sized subunit of a multimeric protein. c. Each band represents a protein subunit with different charge to mass ratio. d. These bands represent two subunits of a heterodimer. e. These bands represent two separate subunits of identical size.

Answers

Answer 1

Answer:

b. Each band represents a different-sized subunit of a multimeric protein.

Explanation:

SDS-PAGE analysis allows the separation and discrimination of proteins according to their molecular weight. The technique separates proteins in an electromagnetic field.

The SDS-PAGE technique makes proteins move in solid support -gel- by the action of the electric field. The distance reached by each protein is proportional to their molecular weight. The protein separation from a mixture results in bands that reflect their size.

SDS is a detergent that denaturalizes the protein and sets a relation charge size, such as that the motion of the protein in the gel is proportional to its weight. The bigger the protein is, the stronger interaction with the polyacrylamide gel there will be.  The speed of displacement in the electromagnetic field is inversely proportional to the protein size.


Related Questions

Why are shadows longer in the morning and evening?
Make sure you give a full, detailed answer, not just one sentence.

Answers

Shadows are longest in the early morning and late afternoon/early evening when the sun appears low in the sky. As the Earth rotates on its axis, the sun hits each location in the morning at an angle. This becomes more vertical as the sun appears to pass more directly overhead around noon.

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What does it mean when a substance sinks in water?

O It is malleable.

O It is not soluble.

Its density is less than water's density.

O Its density is greater than water's density.

Answers

Answer:

Its density is greater than water's density.

Explanation:

If an object is more dense than water it will sink when placed in water, and if it is less dense than water it will float.

describe how scientists would have to use to classify M.smithii into archea domain

pls help asap

Answers

Explanation:

Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla

Answer(Explanation):

Methanobrevibacter smithii is the predominant archaeon in the microbiota of the human gut. M. smithii has a coccobacillus shape. It plays an important role in the efficient digestion of polysaccharides by consuming the end products of bacterial fermentation.

Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla.

The __________ system is made up of the nose, pharynx, trachea, lungs, bronchi, and alveoli. (5 points)

a
circulatory

b
endocrine

c
immune

d
respiratory

Answers

Answer:

respiratory system. it is the system that helps you breathe/lets oxygen in body

Answer:

D. Respiratory

Explanation:

Respiratory is a pertaining to respiration; respiratory assistance. The respiratory system is made up of the nose, pharynx, trachea, lungs, bronchi, and alveoli.

Circulatory is relating to circulatory system or to circulation of the blood.

Therefore, the correct answer is d. respiratory.

What is the name given to your gag reflux organ?

Answers

Answer: pharyngeal reflex

Explanation:

The gag reflex, also known as the pharyngeal reflex, is when your pharynx contracts and your larynx pushes up, contracting your throat.

The afferent limb of the reflex is supplied by the glossopharyngeal nerve (cranial nerve IX), which inputs to the nucleus solitarius and the spinal trigeminal nucleus. The efferent limb is supplied by the vagus nerve (cranial nerve X) from the nucleus ambiguus.

Sustainable agriculture is farming that remains productive and profitable while still conserving
natural resources. Which of the following is an example of a sustainable agriculture practice?
a. Preventing cattle from ever grazing on pastures
b. Removing all manure from pastures where cattle graze
c. Allowing cattle to graze over an entire pasture year round
d. Rotating parts of a pasture on which cattle get to graze throughout the year

Answers

Answer: D rotating parts of a pasture on which cattle get to graze throughout the year.

Explanation:

Dont waste answers Its biology

Answers

Answer:

1. The process shown is Biological Process

2. Planting seeds

3. Lichens, mosses, algae etc.

4. Hardwood forest stage

5. 10 - 20 years

Emma and her younger brother were running a race. After 30 minutes, Emma started feeling pain in her lower leg muscles. a. Suggest the reason for this.

Answers

Answer:

This is due to lactic acid accumulation in Emma's leg muscles.

Explanation:

In the absence of oxygen in the muscle cells, the cell switches from performing aerobic respiration to lactic acid fermentation. The lactic acid fermentation produces lactic acid as a product, which builds up in the muscle cells of the body causing a burning sensation and pain. This often happens during exercises.

This is the cause of Emma's pain in her lower leg muscles. Lactic acid has been formed and accumulated in the muscle cells of her leg during the exercise with her brother.

What kind of disorder might result from a DNA Mutation?

A. none of the above
B. A disorder caused by an event such as an accident
C. An infectious disease caused by a pathogen
D. An inherited genetic disease

Answers

Answer:

some well-known inherited genetic disorders include cystic fibrosis, sickle cell anemia, Tay-Sachs disease, phenylketonuria and color-blindness, among many others. All of these disorders are caused by the mutation of a single gene.

Explanation:

B. A disease that is not contagious is called a noninfectious disease. These diseases are not caused by pathogens. Instead, they are likely to have causes such as lifestyle factors, environmental toxins, or gene mutations. Common types of noninfectious diseases include cancer, diabetes, and immune system diseases.

Where was Flannery O'Connor born? a. Milledgeville b. Savannah C. Andalusia d. Lourdes EL CA VE C​

Answers

The answer is B. Savannah, GA

A student is taking notes about the four steps in hurricane formation. Which of the following should be written as step number 1?

A
tropical disturbance

B
tropical depression

C
tropical storm

D
hurricane

Answers

Answer:

tropical disturbance

Explanation:

I think i am correct let me know if I am wrong

True or false: nutrients are used to break down oxygen and water to release glucose and carbon dioxide

Answers

Answer: it is False

Explanation: because nutrients are not used to break down oxygen and water

But these can be broken down in processes such as PHOTOSYNTHESIS ND CELLULAR RESPIRATION.

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Which of the following words best matches this definition: The process in which the best adapted organisms survive and pass on their traits, while those that are NOT well adapted do not survive. *
A. Natural selection
B. Organic selection
C. Artificial Selection
D. Acquired trait

Answers

Answer:

A. Natural selection

Explanation:

Which of the following statements about natural selection is FALSE? *

a. Natural Selection occurs when organisms with better fitness for an environment survive and reproduce at a greater rate than others.
b. Scientists have evidence that natural selection is continuing to occur even today.
c. Natural selection causes individuals to change the trait they are born with to adapt to a new environment.
d. Natural selection is not a random phenomenon and is a critical part of the theory of evolution.

Answers

Answer:

a.

Explanation:

the process whereby organisms better adapted to their environment tend to survive and produce more offspring. The theory of its action was first fully expounded by Charles Darwin and is now believed to be the main process that brings about evolution.

As
you completed this assignment, the cells in your body were dividing and
the DNA in them was being copied, one _______
at a time.


DNA
Letter
Child
Mutation(used 2 times)

Plz help me

Answers

Answer:

a) DNA

Explanation:

You can quickly take out c and d, because what does a child have to do it with it and mutation isn't going to be used twice.

Letter, I don't know how that could come into a DNA being copied.

Furthermore, the answer is DNA.

"one DNA at a time."

A substance has a half-life of two days. If 10 days have passed, how many half-lives have the substance gone through?
show your work!!

Answers

It’s five isn’t it because Yk 10/2

Half life of the substance will be 5 days  i.e.  10/2=5

The term is most often used in the context of radioactive decay, which occurs when unstable atomic particles lose energy.

What is half life?

It is the time required for a quantity to reduce to half of its initial value.

What is radio active decay?

Radioactive decay is the process by which an unstable atomic nucleus loses energy by radiation

To know more about radioactive decay here

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Which statement most accurately describes a relationship between two
animals in the relationship diagram?
Select one:
A. The turtle and salamander have amniotic eggs.

B.The turtle and opossum have amniotic eggs.

C. The turtle and salamander are warm-blooded.

D. The turtle and opossum are warm-blooded.

Answers

I think the answer is C
I think the answer is C. The the turtle and salamander are warm-blooded

What is a major difference between DNA replication and DNA transcription?
ОА.
ОВ.
DNA replication takes place in the nucleus, while DNA transcription takes place in the cytoplasm.
DNA transcription only occurs in multicellular organisms, while DNA replication occurs in all organisms.
DNA replication involves the nitrogenous base uracil, while DNA transcription involves the nitrogenous base thymine.
RNA molecules produced by transcription are much shorter in length than DNA molecules produced by replication.
Oc.
OD

Answers

Answer:

Transcription copies the DNA into RNA, while replication makes another copy of DNA.

Think about all of the beneficial
adaptations that allow humans
to thrive on Earth. Identify
and explain 3 of these
adaptations.

Answers

Answer: Supplementary angles are two angles whose sum is 180 degrees while complementary angles are two angles whose sum is 90 degrees. Supplementary and complementary angles do not have to be adjacent (sharing a vertex and side, or next to), but they can be. ... The same is true for complementary angles.

Which of the following does NOT play a role in how strong the gravity is between the earth and the moon?
1.the distance from the sun
2.the distance between them
3.the mass of the earth
4.the mass of the moon

Answers

Answer:

the distance from the sun.

Explanation:

one disease that chould happen in resbratory system which can affect homeostasis

Answers

One disease that could happen is the respiratory system which can affect homeostasis is chronic obstructive pulmonary disease (COPD). COPD is a disruption of iron homeostasis in the lungs that is frequently associated with respiratory disease.

One disease that could happen is the respiratory system which can affect homeostasis is chronic obstructive pulmonary disease (COPD). COPD is a disruption of iron homeostasis in the lungs that is frequently associated with respiratory disease.

Use what you have learned about the types of energy that travel from the sun to earth to match each term with is description

Answers

Answer:

Visible light, ultraviolet light, infrared, radio waves, X-rays, and gamma rays.

Explanation:

Visible light, ultraviolet light, infrared, radio waves, X-rays, and gamma rays are types of energy that travel from sun to earth. These types of energy are present in the solar radiation that coming from the sun. Visible light is important for the plants because plants use this visible light in the process of photosynthesis while on the other hand, Ultraviolet light filters by ozone layer that surrounded the earth. This Ultraviolet light is dangerous for humans and animals .

HURRY PLZ How are plant cells related to animal cells? Animal cells have lysosomes and plant cells do not. Both plant and animal cells have lysosomes. Plant cells have lysosomes and animal cells do not. Neither plant nor animal cells have lysosomes.

Answers

Animal cells have lysosomes and plant cells do not

Animal cells have lysosomes and plant cells do not

Which two phases of the moon are expected to come immediately after the phase on August 23?

Answers

Answer:

my name is mister nisegy ok

Explanation:

What usually acts as a heat sink? A. Large bodies of water B. Coastal areas C. Mountains D. Vegetation

Answers

The answer is Coastal areas so B!

Paleontologist have discovered many fossils that document the evolution of birds from dinosaurs that roamed earth long ago what does the fossil record tell us about this change ?

Answers

Answer:

It happened slowly over a long period of time.

Explanation:

Squirrels and birds feed on insects in a forest. Mild temperature and high humidity favors growth of insects.

What will be the effect of decreased humidity on the forest ecosystem?
A.
Population of insects will increase in the forest.
B.
Population of birds will increase in the forest ecosystem.
C.
Competition will increase between squirrels and birds for food.
D.
Squirrels will get more adapted to the changed environmental conditions

Answers

Answer:

competition will increase between squirrels and birds for food.

See would probably be the answer because if humidity decreases then insects will likely decrease also. Because this squirrels and birds will have it harder to find food. Hope this helps :-)

Dose mitochondria transfer things to the cell

Answers

Answer:

Yes, mitochondria transfers energy to the cell.

Explanation:

A cell can be defined as the fundamental or basic functional, structural and smallest unit of life for all living organisms. Some living organisms are unicellular while others are multicellular in nature.

A unicellular organism refers to a living organism that possess a single-cell while a multicellular organism has many (multiple) cells.

Muscle cells use more energy than most other cells in the body. Because of this, muscle cells must have more mitochondria.

Basically, mitochondria is one of the cell organelles found in all living organisms and it is known as the powerhouse. Therefore, mitochondria provides all the energy required in the cell by transforming energy forms through series of chemical reactions; breaking down of glucose into Adenosine Triphosphate (ATP) used for providing energy for cellular activities in the body of living organisms.

Which of the following would MOST LIKELY result in decreasing carbon dioxide levels in a lake?

A) an increase in the amount of precipitation

B) a decrease in competition among producers

C) an increase in the number of fish caught from the lake

D) a decrease in the amount of sedimentation entering the lake

Answers

Answer: C: an increase in the number of fish caught from the lake

Explanation:

An increase in the number of fish caught from the lake would most likely result in decreasing carbon dioxide levels in the lake. Therefore, option C is correct.

What are the effects of carbon dioxide in a lake?

Carbon dioxide can have both direct and indirect effects on a lake's ecosystem. Here are some of the effects:

Acidification: When carbon dioxide dissolves in water, it forms carbonic acid, which can lower the pH of the water.

Oxygen depletion: If carbon dioxide levels are too high, it can lead to oxygen depletion in the water.

Algal blooms: High levels of carbon dioxide can also stimulate the growth of algae in the water. This can result in algal blooms, which can have negative effects on the lake ecosystem, such as oxygen depletion, fish kills, and changes in water clarity.

Thus, an increase in the number of fish caught from the lake would most likely result in decreasing carbon dioxide levels in the lake. Therefore, option C is correct.

Learn more about carbon-dioxide effects, here:

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Gametes (SEX CELLS) have ... A. half the number of chromosomes found in the body cells B. twice the number of chromosomes found in body cells C. two sets of chromosomes

Answers

Answer:

A. Half the number of chromosomes found in the body body cells

Explanation:

Somatic (body cells) have 2 sets of chromosomes, while gametes have 1 set of chromosomes. Sperm has one set, and an egg has one set allowing both to fuse to create a new organism.

Answer:

[tex]\boxed {\boxed {\sf A. \ Half \ the \ number \ of \ chromosomes \ found \ in \ the \ body \ cells}}[/tex]

Explanation:

Gametes are sex cells, such as the egg and sperm. They are used in sexual reproduction to produce offspring.

The sex cells are produced during meiosis. The DNA or genetic material is replicated, then the cells divide twice. After this, four haploid cells are produced with half of the genetic material. For example, human body cells have 46 chromosomes but sex cells have 23 chromosomes.

They contain half the genetic material for an important reason. During fertilization, an egg and a sperm fuse. Each one contributes half of the genetic material from the mother or father to create one complete set for the offspring.

Gametes have half the number of chromosomes in body cells and choice A is correct.

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