how many dna molecules would there be after four rounds of pcr if the initial reaction mixture contained two molecules?

Answers

Answer 1

If two molecules are doubled four times, 32 DNA molecules are created. After 'n' rounds of the polymerase chain reaction, it is possible to produce 2n DNA molecules from a single DNA template molecule.

What is DNA molecules?

Each cycle sees a doubling of the amount of double-stranded DNA, resulting in 2n copies of the DNA after n cycles. The number of DNA molecules (per gram) in your DNA sample can be calculated by multiplying the DNA amount in ng by Avogadro's number, for instance, after 4 cycles you have 16 copies, after 20 cycles you have roughly one million copies, etc (6.022 x 10e23). Each PCR reaction employs two primers, which are created to surround the target region.

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Related Questions

What type of reaction is burning of fuel releases heat and light?

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Combustion is the name for the sort of chemical reaction that occurs when fuel burns, releasing heat and light.

Combustion is a chemical process that takes place between a fuel and an oxidizing agent to create energy, which is often in the form of heat and light. Exergonic or exothermic chemical reactions include combustion.

It is sometimes referred to as burning. Combustion is said to be one of the first chemical processes that humans purposefully regulated. Because the double bond between oxygen atoms in O2 is weaker than single bonds or other double bonds, combustion produces heat.

So, while energy is taken during the reaction, it is released when stronger bonds form to produce carbon dioxide (CO2) and water (H2O). While the fuel contributes to the energy of the reaction, its contribution is minimal in contrast since the energy of the chemical bonds in the fuel is equivalent to the energy of the bonds in the products.

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When the membrane moves, the on the spiral organ hair cells distort because they are anchored by the tectorial membrane.
a. true
b. false

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When the membrane moves, the stereocilia on the spiral organ hair cells distort because they are anchored by the tectorial membrane. Thus, the given statement is true.

What is tectorial membrane?

The tectorial membrane of the inner ear is a ribbon-like strip of extracellular matrix which spirals along the entire length of the cochlea of the ear.

The spiral organ in the ear is also called the organ of Corti, it is defined as the receptor organ for hearing sound, which is located in the cochlea of the ear.

The spiral organ is made up of hair cells which are made up of sensory epithelial cells that act as the sensory receptor of the inner ear.

During transport of sound from ear drum, the movement of the basilar membrane lead to change in the spiral hair cells because they are anchored by the tectorial membrane of the inner ear.

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max is competing in a chin-up competition. what type of muscle contractions are occurring in his biceps muscles, 1) immediately after he grabs the bar , 2) as his body moves upward toward the bar , and 3) when his body begins to descend back toward the floor

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The muscle contractions which occurs in his bicep muscles, immediately after he grabs the bar occur isometrically in the biceps.

The contractions as his body moves upwards towards the bar occurs isotonically and concentrically. The contractions when his body begins to descend back toward the floor causes the biceps to contract isotonically and eccentrically. The contractions which involves shortening of the muscles are referred to as isotonic or concentric contractions. It is felt mainly during squats, bicep curls etc. Eccentric contraction tends to occur when the total length of the muscle increases when tension is produced in them. It is felt mainly during lower body movements such as downhill running, downwards squats etc.

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What is the term for the contractile response of a single muscle fiber to a single muscle impulse?

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Twitch is the term for the contractile response of a single muscle fiber to a single muscle impulse.

Twitch is the sudden involuntary movement in the muscle. This happens when the muscle contract on their own. It is also called muscle fasciculation. There are several reason for twitch like: stress, too much caffeine, a poor diet, exercise, or even side effect of some medicines.

Muscles are the loose tissues in the body that mediate the movement of the body parts. They also provide support of the bones. There are three types of muscles in human body: skeletal, cardiac and smooth muscles.

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RNA polymerase adds ribonucleotides onto the _____ end of a growing rna by forming _____ bonds.

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In order to create an RNA-DNA hybrid of around 8 base pairs in length, RNA polymerase is able to add ribonucleotides that are complementary to the DNA sense strand as the DNA is unwound. As RNA polymerase catalyses the production of phosphodiester bonds between the ribonucleotides, a developing RNA chain is generated.

Does the 3 end get added to by RNA polymerase?

RNA polymerase, which creates a complementary strand of RNA using a single-stranded DNA template, is the primary enzyme involved in transcription. Each new nucleotide is added to the 3' end of the strand as RNA polymerase constructs an RNA strand in the 5' to 3' orientation.

Do ribonucleotides get added by RNA polymerase?

Based on complementary base pairing, RNA Polymerase adds ribonucleotides to the template strand to produce an mRNA. The promoter region of the DNA strand is where the sigma protein initially breaks apart the double helix. The RNA polymerase then passes the DNA template strand through it.

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a student proposes that left-handedness is a recessive trait. a survey of a class of 36 students finds that 27 (0.75) are right-handed and 9 (0.25) are left-handed. using the hardy-weinberg formula, what would the expected genotype and allele frequencies be in this theoretical population

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0.25 right-handed homozygous, 0.50 heterozygous, and 0.25 recessive homozygous for a 0.5 allele frequency for each allele.

The genotype of an organism is its complete set of genetic material. Genotype can also be used to refer to the alleles or variants an individual carries in a particular gene or genetic location.

The number of alleles an individual can have in a specific gene depends on the number of copies of each chromosome found in that species, also referred to as ploidy.

In diploid species like humans, two full sets of chromosomes are present, meaning each individual has two alleles for any given gene. If both alleles are the same, the genotype is referred to as homozygous. If the alleles are different, the genotype is referred to as heterozygous.

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many neurons of joints help to inform the brain about the activity of the joint, including pain or position of the joint. these types of neurons are classified as _____________________ neurons.

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Many neurons of joints help to inform the brain about the activity of the joint, including pain or position of the joint. These types of neurons are classified as afferent neurons.

Neurons are the nerve cells that are the building blocks of the nervous system. These cells perform the function of transmission of impulses to and from the brain. The neurons are also the largest cell in the body. The structure of neurons is composed of cell body, dendrites, axon and axon terminal.

Afferent nerves or neurons are the cells that collect the information from the outside world and bring it as signal to the brain. Their path begins from the sensory receptors and ends to the different parts of the brain.

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What environmental parameters would need to be monitored as DDTmoves up the food web of an ecosystem?How much DDT is in the ecosystem

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DDT is a persistent organic pollutant that is known to move up the food web of an ecosystem when it is released into the environment.

The Impact of DDT on Ecosystems: Monitoring Environmental Parameters Up the Food Web

Soil and water levels of DDT and its metabolites.Air quality of the environment.Concentration of DDT in the tissues of top predators.Abundance and diversity of plants, insects, and animals in the food web.Concentrations of DDT in forage and prey items.Concentrations of DDT in the eggs of birds and other species.Reproductive success of species in the food web.Incidence of disease in species in the food web.

It is important to monitor the levels of DDT and its metabolites in the soil, water, and air, as well as its concentrations in the tissues of top predators, in order to understand how it moves through the food web and affects the environment.

Additionally, monitoring the abundance and diversity of species, concentrations of DDT in forage and prey items, and concentrations in the eggs of birds and other species can provide important insights into the effects of DDT on the environment.

Finally, it is also important to monitor the reproductive success of species in the food web and the incidences of disease in order to gain an accurate understanding of the impacts of DDT on the environment.

DDT in the ecosystem

The amount of DDT in the ecosystem depends on the amount that has been released into the environment, as well as the rate of its breakdown and transport. DDT levels can be measured in the soil, water, air, and tissues of top predators, as well as in forage and prey items, eggs, and other biota.

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Reactive oxygen intermediates and reactive nitrogen intermediates produced by phagocytic cells are effective in killing invading microorganisms. True /False

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The statement reactive oxygen intermediates and reactive nitrogen intermediates produced by phagocytic cells are effective in killing invading microorganisms is true.

An enzyme called a NO synthase converts L⁻ arginine into the reactive nitrogen intermediate (RNI) nitric oxide (NO. ), which is then followed by secondary reactions that result in the formation of the hazardous intermediates nitrite (NO₂⁻ and nitrate. But nitrite also has hazardous qualities. The successive 1-electron reduction products of O₂ on the way to producing water are known as reactive oxygen intermediates (ROI) (see inset). Hydrogen peroxide (H₂O₂), the hydroxyl radical (•OH), and the superoxide anion radical are examples of ROI.

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a shark eats a penguin that has recently been feeding on some krill. the penguin has 100 joules worth of energy, but the shark ultimately gains only about 10 joules. where does the rest of the energy go? (2 points)

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Answer: answer below

Explanation: A shark eats a penguin that has recently been feeding on some krill. The penguin has 100 joules worth of energy, but the shark ultimately gains only about 10 joules. Where does the rest of the energy go? It is used for cell functions or transformed to heat energy.

Is fire thermal or chemical energy?

Answers

Fire is a chemical process that produces carbon dioxide and water from a fuel and oxygen. It is an exothermic process, which means it generates heat.

Fire is a chemical process that requires three fundamental components: oxygen, fuel, and heat. Because oxygen is present almost everywhere on Earth that is not buried or underwater, and many objects are made of fuel (everything that burns), those items are just waiting for enough heat to cause them to burn.

Fire needs heat to burn, but it also generates heat. That is how fire can both maintain itself and spread. When one thing catches fire, it continually radiates heat to its surrounds.

If an object made of fuel (such as wood, paper, or cloth) is nearby and warms up sufficiently, it will begin to burn. Every flammable object that is sufficiently heated in an oxygen-rich atmosphere will catch fire.

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check all that are true statements regarding the effects of the parasympathetic division.

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The true statements about effects of the parasympathetic division are:

(1) The lack of divergence in the preganglionic axons prevents the mass activation that is seen in the sympathetic division.

(3) Parasympathetic activity can affect only one group of organs without affecting all other organs.

(4) The parasympathetic division is most active when the body must process nutrients and conserve energy.

Parasympathetic is the type of autonomic nervous system that functions after the body's flight and fight response to calm down the vitals of the body. It relaxes the body and brings it back to its normal state.

Axons are the part of the nerve cells that are elongated in structure. Its function is the carry the signals from one neuron to another. they are very thin in shape.

The given question is incomplete, the complete question is:

Check all that are true statements regarding the effects of the parasympathetic division.

The lack of divergence in the preganglionic axons prevents the mass activation that is seen in the sympathetic division.The effects of the parasympathetic division tend to be widespread. Parasympathetic activity can affect only one group of organs without affecting all other organs.The parasympathetic division is most active when the body must process nutrients and conserve energy.

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which of the following is accurate about resistance to rifampin? which of the following is accurate about resistance to rifampin? a bacterium that is resistant to the tb drug rifampin produces an rna polymerase enzyme that transcribes more slowly in the presence of rifampin than a bacterium that is not resistant. a person who is resistant to the tb drug rifampin produces an rna polymerase enzyme that transcribes more slowly in the presence of rifampin than a person who is not resistant. a bacterium that is resistant to the tb drug rifampin produces an rna polymerase enzyme that transcribes more efficiently in the presence of rifampin than a bacterium who is not resistant. a person who is resistant to the tb drug rifampin produces an rna polymerase enzyme that transcribes more efficiently in the presence of rifampin than a person who is not resistant.

Answers

Rifampin Resistance Development in Escherichia coli and Mycobacterium smegmatis as a Result of Poor Drugs

Infectious disorders like tuberculosis, which require months of treatment with rifampin and other medications, are made more difficult to treat by subpar pharmaceuticals. Rifampin resistance is a serious issue for the treatment of tuberculosis. Antibiotic resistance is known to be selected for by subtherapeutic doses of medication, but it is uncertain how drug breakdown products affect the evolution of resistance.

Here, we show that substandard medications that have active pharmacological ingredients that have degraded select for gene changes that give resistance to recommended medications. By serially cultivating bacteria in the presence of the rifampin breakdown product rifampin quinone, we were able to produce drug-resistant Escherichia coli and Mycobacterium smegmatis strains. With certain populations exhibiting no growth inhibition at maximum rifampin concentrations, strains resistant to rifampin quinone developed cross-resistance to the common antibiotic rifampin.

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which disorder has traits that are found primarily under neuroticism in the five-factor model of personality?

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Borderline personality disorder has traits that are found primarily under neuroticism in the five-factor model of personality.

Borderline personality disorder is a mental state disorder that impacts the method you think that and feel regarding yourself et al, inflicting issues functioning in way of life. It includes self-image problems, issue managing emotions and behavior, and a pattern of unstable relationships.

The five-factor model of personality breaks temperament down into 5 components: Agreeableness, Conscientiousness, extroversion, Openness, and Stress Tolerance. temperament tests that are supported this model measure wherever a personal lies on the spectrum of every of the 5 traits.

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you are working at a hominin fossil site in east africa when you come across a skull. you note that the brain size is small compared to modern humans, but the canines are nonhoning. additionally, the face seems very flat. to which species does this skull most likely belong?

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The skull is most likely to belong to the species of Australopithecus africanus and Australopithecus robustus.

The first was a predecessor of the homo sapiens, the second shared its time on earth with early humans. Among the inscribed locations is the Taung Skull site, where in 1924 the first example of the Australopithecus africanus was discovered.

   The oldest fossils date to 3.5 million years ago. The oldest stone tools have been discovered here too, and the oldest evidence of controlled use of fire. Hundreds of specimens of hominids and early humans have been found, these high numbers contribute to the value of these sites.

   According to the given conditions, it was noticed that the brain size is small compared to other humans. Also, according to the information hominids were the first ancestors to human and as the evolution took place it increased.

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regarding gi function, which of the following best describes the actions of atropine? a. atropine enhance the release of gastric juices. b. atropine is a competetive antagonist of cholinergic muscarinic post- synaptic receptors. c. atropine is a competetive antagonist of adrenergic post- synaptic receptors. d. atropine is secreted by chief cells in the stomach.

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The simultaneous induction of two second messenger reactions—adenylyl cyclase inhibition, which reduces cAMP accumulation, and stimulation of phospholipase C (PLC), which hydrolyzes phosphoinositides to produce inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol—is a characteristic of mAChR activation in gastrointestinal smooth muscle (DAG). A number of proteins are phosphorylated as a result of IP3 releasing Ca2+ from intracellular reserves and DAG activating protein kinase C (PKC) [1,9,15]. Additionally, intricate reciprocal regulation of second messenger pathways is shown by muscarinic subtypes.

In ileal longitudinal muscles from mAChR-KO and wild-type mice, Sakamoto et al. (2007) [23] investigated the effects of the powerful non-subtype-selective muscarinic agonist carbachol on buildup of cAMP evoked by the -adrenoceptor agonist isoprenaline, which activates adenylyl cyclases. A greater amount of isoprenaline-stimulated cell death was inhibited by carbachol (1 M).

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why are proteins treated with ionic detergent (sds), reducing agents (dtt), and heat before sds-page?

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These processes get the protein samples ready for effective gel migration. Large biomolecules and macromolecules known as proteins are made up of one or more extended chains of amino acid residues.

Large biomolecules and macromolecules known as proteins are made up of one or more extended chains of amino acid residues. Among the many tasks that proteins carry out in living things include catalyzing metabolic processes, replicating DNA, reacting to stimuli, giving cells and organisms structure, and moving molecules from one place to another. The primary way that proteins differ from one another is in the order of their amino acids, which is determined by the nucleotide sequence of their genes and typically causes a protein to fold into a certain 3D structure that controls its activity. A polypeptide is an ordered sequence of amino acid residues. At least one lengthy polypeptide is present in every protein. Less than 20–30 residue polypeptides are frequently referred to as peptides and are rarely thought of as proteins. Peptide bonds and nearby amino acid residues hold the individual amino acid residues together. The sequence of a gene, which is encoded in the genetic code, determines the arrangement of amino acid residues in a protein.

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What is the importance of positive parent involvement regarding sexuality?

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The important is that sexuality is in the Parental tracking is the attention and supervision in their offspring's behavior.

Well-monitored teenagers document much less sexual activity, fewer sexual partners, and expanded use in comparison to poorly-monitored adolescents. Parent involvement motivates kids to learn, main to better grades.

The stage of involvement is critical in generating a excessive effect at the student's performance. The better the diploma of parental involvement, the better the effect at the toddler's educational achievement. Research has discovered that properly determine-toddler communique round sexuality has many effective results for teens – inclusive of assisting them defend their personal sexual health.

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What do you mean by polypeptide and amino acids?

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Polypeptides make up proteins, and amino acids are the fundamental building blocks of polypeptides.

A peptide is a group of two or more amino acids bound together by peptide bonds, while a polypeptide is a chain of several amino acids. A protein is made up of one or even more polypeptides. Proteins are therefore big strands of amino acid residues joined by peptide bonds.

Through a series of peptide bonds, amino acids come together to form polypeptides, another term for proteins. The polypeptide will subsequently fold into a particular shape depending on the linkages between both the side chains of the amino acids.

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how does the models support the idea that genetic mutations may arise from environmental factors?

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The models support the idea that genetic mutations may arise from environmental factors in environmental factors, genetic mutations, and so on.

Firstly, the models suggest that environmental factors may cause gene mutations through direct damage to the DNA. This direct damage can be caused by several external sources, such as ultraviolet radiation, chemicals, and toxins.

The models also support the idea that genetic mutations can arise due to indirect environmental factors. These can include changes in the cell’s environment, such as changes in temperature, pressure, or nutrient availability. These environmental changes can affect the expression of certain genes, leading to mutations.

The models also suggest that environmental factors can cause mutations through epigenetic changes. Epigenetic changes are changes in gene expression that can be caused by environmental factors such as diet, stress, and toxins. These changes can lead to genetic mutations, which can then be passed on to future generations.

Finally, the models suggest that genetic mutations can be caused by changes in the genetic code itself. This can happen when a mutation occurs in the DNA sequence of a gene, causing it to become different from its original state. This type of mutation is called a point mutation and can be caused by factors such as radiation or chemicals.

Overall, the models suggest that environmental factors can cause genetic mutations in a variety of ways. By understanding how these mutations occur, scientists can better understand the effects of environmental factors on our health and the health of future generations.

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deep homology of homeotic genes is also seen in plants. for example, the mads-box genes have been used to work out phylogenetic relationships within flowering species of plants. what was also documented in these studies across many plant lineages?

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The MADS-box homeotic genes are also instrumental in nonflowering plant species, where they are involved in developmental pathways in leaf and root systems.

The development of anatomical traits in a range of animals, such as echinoderms, insects, mammals, and plants, is governed by homeotic genes. Homeotic genes typically express transcriptional factor proteins, which have an impact on development by regulating downstream gene networks involved in body patterning. Displaced body parts result from errors in the homeotic genes, such as antennae growing on the fly's back instead of its head. Mutations with ectopic structure-growing are frequently deadly. There are several different subgroups that make up the homeotic gene set. They contain a number of segmentation-related Hox and ParaHox genes. Hox genes are present in all bilateral animals, including Drosophila (where they were first discovered) and humans.

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Drooping of the upper eyelid, and double vision are potential symptoms of damage to the ______ nerve.
Select one:
a. oculomotor
b. trigeminal
c. optic
d. trochlear
e. abducens

Answers

Damage to a oculomotor nerve may manifest as double vision and drooping of a upper eyelid.

What in the name of god is speed within physics, and how is it measured?

The rate at which a location shifts over time is what constitutes speed. It has a time-based dimension of distance. The Vernier scaling of speed is comprised of the basic units of time and distance. Meters per second (m/s) is the unit of velocity in the metric system.

The definition of speed

What is speed, exactly? the rate of a directionally changing object's location. The ratio of the length to the time it has taken to travel a distance is known as speed. Speed is in fact a scalar number, having only a direction and no magnitude.

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which glands secrete their product by way of a duct directly onto an epithelial surface?

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The answer is Exocrine Glands

Animals breathe in air containing oxygen and breathe out air containing less oxygen. The consumed oxygen is used: a. in the glycolysis pathway. b. in the citric acid cycle. c. as an electron acceptor in the respiratory electron transport chain. d. in photosynthesis. e. in the conversion of pyruvate to acetyl Co-A.

Answers

The consumed oxygen is used c. as an electron acceptor in the respiratory electron transport chain.

In the field of biology, the electron transport chain can be described as a process where electrons are taken from compounds that are electron donors to compounds that are electron acceptors. The electron transport chain is a series of redox reactions that occur in the mitochondrial matrix.

Animals breath in the air and the consumed oxygen serves as an electron acceptor in the respiratory electron chain process. The consumed oxygen is changed into an ion by accepting two electrons and circulating it via the electron transport chain.

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When the lobes of two p orbitals are in the same phase and overlap side-by-side, the orbital that is formed has a _____ dissociation energy compared to the σ∗ molecular orbital formed from the out-of-phase direct overlap of the two p orbitals.

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When the lobes of two p orbitals are in the same phase and overlap side-by-side, the orbital that is formed has a less dissociation energy compared to the σ∗ molecular orbital formed from the out-of-phase direct overlap of the two p orbitals.

Orbital is the location of an electron in the three dimensional space around an atom. Thus, orbital is simply the address of electron. There are 4 orbitals that an electron can occupy: s, p, d and f. Besides location, orbital also describes the wave nature of electron.

Dissociation energy is defined as the energy released when a bond is broken between two atoms or molecule. Since energy is released in the process, it is an endothermic reaction.

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chaparral vegetation occurs around much of the central valley of central and southern california. this biome is very similar to that found

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Chaparral vegetation occurs around much of the central valley of central and southern California. This biome is very similar to that found: in the Mediterranean region.

Chaparral vegetation is composed of the shrubs that have hard stems and leathery leaves. The climate here is comprised of dry hot summers and mild wet winters. Chaparral can be categorized as woodland but rich in shrubs.

Biome is defined as an area that has its unique species of plants and animals that reside their due to the suitable physical factors like the soil, climate, etc. Biome is also known by the name major life zone.

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Which term best describes a chemical process in which the temperature
increases?
OA. Endothermic
OB. Closed system
C. Combustion
D. Exothermic

Answers

Answer: I believe the anser is D( exothermic

Explanation: Exothermic is when the temperature of the reaction mixture increases.

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the growth hormone axis contains at least one example of a negative feedback loop. t or f

Answers

It is true that the growth hormone axis contains at least one example of a negative feedback loop.

What is negative feedback loop?

Growth hormone secretion is part of a negative feedback loop involving IGF-I. High blood levels of IGF-I causes decreased secretion of growth hormone by directly suppressing the somatotroph and also by stimulating release of somatostatin from the hypothalamus.

An important example of a negative feedback loop is in control of thyroid hormone secretion. The thyroid hormones that are thyroxine and triiodothyronine are synthesized and secreted by thyroid glands and affects metabolism throughout the body.

Example of biological negative feedback is : Human body temperature.- Hypothalamus of a human responds to temperature fluctuations and also responds accordingly.

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dna fragments can be separated out by size using gel electrophoesis. what property of dna allows this process to occur?

Answers

Because the DNA molecule is negatively charged, an electric field can draw it through the gel. This property of DNA allows this process to occur.

What is gel electrophoresis? Describe how this is used to separate and detect DNA fragments.

The technique of gel electrophoresis is used to separate mixture components according to their sizes. DNA, RNA, or even a combination of proteins could make up the mixture. On the electrophoresis plate, a gel matrix made of a suitable material is spread (for example, agarose gel). A grove in the gel is loaded with the mixture, and an electrical field is then applied to it. Depending on the size and charge of their molecules, the particles in the mixture move away from the initially charged end and toward the oppositely charged end. It has been observed that smaller particles travel more quickly and farther from the initial position.

What is DNA?

The molecule that is found inside cells that carries the genetic material necessary for an organism to develop and function. This knowledge can be passed on from one generation to next thanks to DNA molecules.

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What is the definition of a common ion as it applies to Le Chatelier’s principle?
an ion that is found in a wide variety of compounds
an ion that is present in every sample of pure water
an ion that is found dissolved in most aqueous solutions that are widely used in labs
an ion that is present in an equilibrium system and a compound added to the system

Answers

Answer: answer is below

Explanation: Answer: D) an ion that is present in an equilibrium system and a compound added to the systemExplanation: Any change in the equilibrium is studied on the basis of Le-Chatelier's principle.This principle states that if there is any change in a equilibrium reaction, the equilibrium will shift in a direction so as to minimize the effect.Thus when a common ion is introduced to an equilibrium reaction, the equilibrium will shift in a direction where the concentration of common ion is decreasing.For example: In a equilibrium reaction for dissociation of acetic acid:If HCl is introduced wth  as common ion.The equilibrium will shift in backward direction where the concentration of  is decreasing.

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