A scientist runs an SDS-PAGE experiment two separate times on a protein with a molecular weight of 200 kD. For the first experiment he treats the protein with SDS only and for the second experiment he treats the protein with both SDS and a reducing agent. If the first gel yields one band showing a molecular weight of 200 kD and the second run yields one band showing a molecular weight of 25 kD, the researcher should conclude that

Answers

Answer 1

Answer:

This protein consists of 8 subunits of equal size linked by disulfide bonds

Explanation:

The sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) is a technique widely used in molecular biology laboratories to separate proteins with molecular weights between 5 and 250 kDa. SDS is an anionic detergent used to denature proteins before electrophoresis. SDS can denature proteins by altering non-covalent bonds such as hydrogen, hydrophobic and ionic interactions, but they cannot cleave disulfide bonds. In this case, reducing agents (e.g.,  β-mercaptoethanol or dithiothreitol) have been used to cleave disulfide bonds.


Related Questions

What is the most common stimulant drug used today?
A.Heroin
B.Cocaine
C.Caffeine
D.Alcohol

Answers

Answer:

B. cocaine

Explanation:

Should people try to save wild populations of the Asian elephant? (Complete sentences says my teacher :/ )

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Yes, people should try to save Asian elephants. They are hunted and killed for their tusks. These parts of their body are used for medicinal reasons. Many people “save” elephants by trimming off their tusks, making it so that killing the animal would be meaningless.

(Hope this is what you wanted, I learned this in a documentary forever ago.)

Short explanation: Elephants are in danger due to poachers, they hunt them for their tusks.

Don’t know how much you needed but here you go

Pls someone help its a very easy question also don’t worry about the dot I accidentally did that

Answers

The 2 one is the right answer

If the bird population is directly proportional to the average monthly temperature, which month would have the LOWEST temperature?

Question 1 options:

8- August


12-December


1- January


10 October

Answers

Answer:

December

Explanation:

If the bird population is directly proportional to the average monthly temperature, 12 december would have the lowest temperature.

What is population?

The population of an organism can't survive if it exceeds it's carrying capacity. If animal population increases the population that eat that animal might also increase

The causes that affect population growth animals that if there are more plants than usual in area, populations of animals that eat that plant may increase. If animal population increases the population that eat that animal might also increase.

The effects of animals population can be dangerous and when population exceed the carrying capacity of their environment, animal suffer of malnutrition and disease. When predators became scarce due to over population, the weekar species of animal start or die in other ways .

Therefore, If the bird population is directly proportional to the average monthly temperature, 12 december would have the lowest temperature.

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Two ways that hostility can affect the body are through stress on the heart and Select one: a. increased pulmonary function. b. reduced pulmonary function. c. decreased inflammation. d. increased lymphocyte activity.

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Answer:

sorry hindi ko po alam nyan pag alam ko sasabihin ko po sa inyo promise po nyan

Which sex would you expect to be the choosiest?

Answers

Answer:

Feamles but it depends what they're choosing from

Explanation:

I don’t really get the question so I’m guessing you’re saying which gender is the pickiest in terms of looks wise. I would say guys because most of them look at physical appearance and overall is very choosy for everything. And no not all are like that they’re girls like that too

ability of a cell to form the whole organism​

Answers

Answer:

Totipotency

Explanation:

Totipotency can be regarded as

capability of single cell to divide,develop as well as to form differentiated cells in a living body. This could be found in Meristematic plant cells since they posses the ability to develop into complete plants. Totipotent cells can be formed during sexual and asexual reproduction and these could include spores and zygotes. Some organism can experience totipotency in the sense that cells can dedifferentiate as well as regaining totipotency. Instance of this is plant cutting which used in growing an entire plant.

I NEED HELP ON BOTH ANSWER AS QUICKLY AS POSSIBLE!!

Answers

In a point mutation, only one nucleotide is changed.

In a chromosomal mutation, the whole chromosome is changed.

The corresponding mutation types and sequences are given below:

Deletion: ATT GGA TA

Substitution: ATT ACG ATA

Insertion: ATA TGC GAT A

Which of these genotypes represents a carrier?
Аа
aa
XXY
AA

Answers

Answer:

Aa

Explanation:

from what I can remember from 7th grade science

practice: density calculations

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What about it?
Tell us more

PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

Answers

Answer:

Relative Dating of rock layer

it’s the first answer, they can predict how long the earth has been here by examining the rock layers

3. Microphages are a type of white blood cells and part of the immune system's response to defend the body against viruses. What do microphages do?​

Answers

Macrophages are specialised cells involved in the detection, phagocytosis and destruction of bacteria and other harmful organisms. In addition, they can also present antigens to T cells and initiate inflammation by releasing molecules (known as cytokines) that activate other cells.

In order to assign a Richter magnitude to an earthquake event, a scientist would have to measure the _________ waves on a seismogram.
a. secondary
b. surface
c. primary

Answers

Primary waves on a seismograph

How does gene regulation relate to organism complexity?

Answers

Answer:

Organisms that are more complex, such as eukaryotes, have cells that are specialized for specific functions. Gene regulation needs to be more complex to produce these specialized cells for complex organisms.

Hope this helps!

More complex organisms such as eukaryotes have cells that are specialized for specific functions where gene regulation needs to be more complex for complex organisms to produce these specialized cells.

What is Gene regulation?

The process of turning genes on and off, defined as gene regulation which is an important part of normal development. For example, genes turn on and off in different patterns during development to make a brain cell look and function differently than a liver cell or a muscle cell.

Gene expression is controlled at two levels which is transcription which is controlled by limiting the amount of mRNA that can be produced from a particular gene and the second control is through post-transcriptional events which allow the translation of mRNA into protein.

Thus, more complex organisms such as eukaryotes have cells that are specialized for specific functions where gene regulation needs to be more complex for complex organisms to produce these specialized cells.

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Which term indicates that a single gene has two or more alleles present in a population?

A. Genotype
B. Point mutation
C. Polymorphism
D. Pleiotrophy

Answers

Urm I think the answer is C

Genetic polymorphism is the existence of multiple alleles of a single gene.

What are genes?

Genes are regions of DNA which code for a particular protein or any other gene product.

Genes can exists as a single copy or as multiple copies.

Alleles are alternate forms of a particular gene.

A single gene that has two or more alleles present in a population is known as genetic polymorphism.

Therefore, multiple alleles of a single gene is known as genetic polymorphism.

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how and why does DNA change during meiosis

Answers

Answer:

When recombination occurs during meiosis, the cell's homologous chromosomes line up extremely close to one another. Then, the DNA strand within each chromosome breaks in the exact same location, leaving two free ends. Each end then crosses over into the other chromosome and forms a connection called a chiasma.

Explanation:

PLS ANSWER QUICK! I WILL GIVE POINTS, ITS JUST A YES OR NO QUESTION!


In two or more complete sentences, describe a scenario where human activities are related to an increase or decrease in biodiversity in an ecosystem.

I WAS THINKING OF WRITING ABOUT THE DEFORESTATION IN THE AMAZON, HOW THAT CAN KILL THE ANIMALS. OBV IF THE ANIMALS ARE DEAD THAT CAUSES A DECREASE IN BIODIVERSITY. IS THIS RIGHT?

Answers

Answer:

Yes?

Explanation:

What sphere refers to water and ice?
a. atmosphere
b. hydrosphere
c. lithosphere
d. biosphere

Earth's outermost sphere is called the
a. biosphere.
b. atmosphere.
c. lithosphere.
d. hydrosphere.

What part of Earth contains rocks?
a. Biosphere
b. Hydrosphere
c. Atmosphere
d. Lithosphere

What of Earth's spheres is composed of a mixture of gases?
a. mesosphere
b. hydrosphere
c. atmosphere
d. biosphere

What is Earth's hydrosphere?
a. the gases in the air
b. the solid, rocky part of Earth
c. all of the water on the planet
d. the study of Earth's atmosphere​

Answers

1) It should be the crysophere but since it is not I am not sure I am going to with hydrosphere
2)B
3)D
4)C
5)C

The Minimal Chromosome To enable it to be transmitted intact from one cell generation to the next, the linear DNA molecule of a eukaryotic chromosome must have appropriate nucleotide sequences making up three special kinds of regions: origins of replication (at least one), a centromere, and two telomeres. What would happen if such a chromosomal DNA molecule somehow lost: a) all of its origins of replication

Answers

Answer:

The chromosome wouldn’t be able to replicate

Explanation:

Without an origin of replication, the chromosome could not be copied prior to mitosis.

PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

Answers

The answer is C. Good luck

Answer:

radioactive dating

Explanation:

It's the same thing as radiometric dating

Practice Problem #1
DNA
TAC
CGC
TCC
GCC
GTC
GAC
AAT
ACC
ACT
???
mRNA
???
???
???
???
???
???
???
???
???
???
???
???
???
???
???
???
TRNA
???
???
amino
acids
???
???
???
???
???
???
???
???

Answers

Answer:

Base Substitutions. Single base substitutions are called point mutations, recall the point mutation Glu -----> Val which causes sickle-cell disease.

Deletions. ...

Insertions.

How many atoms of the element hydrogen are there for each atom of oxygen in the compound we know of as 'water'?
a. 8
b. 4
c. 1
d. 2

Answers

Answer:

d

the formula is H2O

Explanation:

What sort of receptor do skeletal muscle cells use to receive signals from somatic lower motor neurons?
a. Nicotinic
b.Muscarinic
c.Adrenergic
d.Mechanoreceptive calcium channels

Answers

Answer:

C

Explanation:

Skeletal muscle cells use Adrenergic receptor to receive signals from somatic lower motor neurons ; ( C ) Adrenergic

Skeletal muscles are organs of the muscular system found in vertebrates. they are attached to the skeletal bones through tendons. they all posses the longest of all muscles cells as well.

Skeletal muscles receive signals from somatic lower motor neurons through the Adrenergic receptor found in all cell and tissues .

Hence we can conclude that skeletal muscle cells use Adrenergic receptor to receive signals from somatic lower motor neurons.

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The hormone insulin is secreted by the pancreas. Which activity is MOST likely to
trigger an increase in the levels of insulin circulating in the blood?
O A. driving
B. eating
O C. jogging
OD. sleeping

Answers

Answer:

Eating

Hope this helps! <3

What is the primary mechanism associated with the role of fluoride in prevention of dental caries?​ a. ​Fluoride increases calcium absorption, which increases crystal formation of teeth b. ​Fluoride increases salivation which aids in the removal of bacteria and the foods needed to sustain bacterial growth c. ​Decay is reduced due to the inhibitory effects of fluoride on growth of bacteria on the teeth d. ​Fluoride becomes incorporated into the crystalline structure of teeth, making them less susceptible to decay e. ​Decay is inhibited due to neutralization of organic acids produced by bacteria on the teeth

Answers

Answer:

d. ​Fluoride becomes incorporated into the crystalline structure of teeth, making them less susceptible to decay

Explanation:

Dental caries results when cariogenic (caries-causing) bacteria metabolize carbohydrates or sugars present in the mouth for their energy needs, producing organic acids as byproducts, thus lowering the pH of the plaque biofilm.

The hydroxyapatite of tooth enamel is composed mainly of phosphate ions (PO₄³⁻) and calcium ions (Ca⁺). Under normal conditions, there is a stable equilibrium between the calcium and phosphate ions in saliva and the crystalline hydroxyapatite. At low pH (acidic conditions), there is a shift in equilibrium resulting in the dissolution of hydroxyapatite from tooth enamel, a process known as demineralization. A reverse process called remineralization (the process of incorporation of minerals in tooth enamel) occurs when the saliva, acting as a buffer elevates pH. When equilibrium is shifted to favor continued demineralization, dental caries results.

However, when fluoride is present in saliva, fluorapatite, rather than hydroxyapatite, forms during the remineralization process as fluoride ions replace hydroxyl groups in the formation of the apatite crystal lattice.

Fluorapatite is less soluble than hydroxyapatite making it more resistant to demineralization, even under conditions of low pH. Thus, fluoride acts as an effective anticaries agent.

pulled upwards in the trunk of a tall tree Ichestue
S. Give three reasons to explain why transpiration occurs mainly through leaves
6. Explain how sunken stomata lower the rate of transpiration
11
with
ftri​

Answers

Answer:

Due to stomata.

Explanation:

Transpiration occurs mainly through leaves because of the presence of stomata n the lower side of the leaves. Transpiration refers to the loss of water from the body of plants so leaves has small pores which is used by the plant to regulate internal temperature.  Sunken stomata lower the rate of transpiration because it is not directly exposed to the surface which protects the escaping water vapor due to air.

What is the argument against taking persistent vegetative state patients off of life support?

Answers

Answer:

An argument could be that there have been many instances where people on life support or in a vegatative state have 'woken' up and gotten better after many years. So, taking someone off life saving machines could be taking away their chance of getting better and their chance of surviving. Since the person is not dead, some may even see it as murder.

Explanation:

umm I tried <3

How do bacteria multiply again? Use the key terms genetic information, DNA, split in two, and exponential growth.

Answers

Answer:

Bacteria are asexual. This means that they are not like us, as they do not need a partner to multiply. A bacterium can become two bacteria all by itself. Then those two bacteria can each multiply again on their own and so, they become four bacteria.

Explanation:

Hope this helped Mark BRAINLEST!!!

The role of the RNA-induced silencing complex (RISC) in RNA interference is to cleave tRNAs to slow the rate of translation. disrupt gene expression by cleaving mRNAs that are complementary to the guide strand of the RNA duplex that was introduced. cleave the introduced RNA duplex. disrupt gene expression by interfering with mRNA export to the cytoplasm. create a passenger strand to bind to the mRNA and keep the mRNA from being translated.

Answers

Answer:

disrupt gene expression by cleaving mRNAs that are complementary to the guide strand of the RNA duplex that was introduced

Explanation:

The RNA-induced silencing complex (RISC) is a ribonucleoprotein complex composed of proteins that associate with regulatory small non-coding RNAs (sncRNAs) such as, for example, microRNAs (miRNAs) and small interfering RNAs (siRNAs) in order to regulate gene expression by the RNA interference (RNAi) pathway. These sncRNAs (around 18-30 nucleotides in length) are complementary or nearly complementary to messenger RNAs, thereby they can bind to target mRNA sequences in order to trigger RNAi via mRNA degradation, translational repression, etc. The core component of the RISC complex is an evolutionary conserved Argonaute (Ago) protein that cleaves target mRNAs.

PLSS HELP

Look at the picture and answer the question(s) that follow.

Which of these strata are MOST likely the oldest?
-J and E
-K
-A and F
-D and E

Answers

I think it's J and E
J and E are the oldest